Linked Life Data

21112994

Source:http://linkedlifedata.com/resource/pubmed/id/21112994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 24
pubmed:dateCreated
2010-11-29
pubmed:abstractText
To protect cells from the damaging effects of environmental stresses, all organisms possess a universal stress response involving upregulation of heat shock proteins (Hsps). The mechanisms underlying chilling injuries and the subsequent recovery phase are only beginning to be understood in insects. Hsp22 and Hsp23 are both upregulated during the recovery from prolonged chill coma in Drosophila melanogaster. This prompted us to investigate the functional significance of these modulations by testing whether expression of these two small Hsps is necessary for recovery after cold stress. We used the GAL4/UAS system to separately knock down expression of Hsp22 and Hsp23, and assayed three aspects of recovery performance in transgenic adults that had undergone 12 h of chill coma at 0°C. The time to recover (short-term recovery) and mobility parameters (medium-term recovery) were significantly impaired in the transgenic flies in which Hsp22 or Hsp23 was suppressed. Our findings show that both Hsp22 and Hsp23 play important roles in the recovery from chill coma in adult males, and suggest that these contribute to adaptive responses to fluctuating thermal conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1477-9145
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4146-50
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster.
pubmed:affiliation
Earth and Life Institute, Biodiversity Research Centre, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium. herve.colinet@uclouvain.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't