Linked Life Data

21112325

Source:http://linkedlifedata.com/resource/pubmed/id/21112325

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pubmed-article:21112325pubmed:issue3lld:pubmed
pubmed-article:21112325pubmed:dateCreated2011-2-21lld:pubmed
pubmed-article:21112325pubmed:abstractTextCpa135 is a multidomain antigenic protein secreted at the sporozoite stage of the Apicomplexa protozoan Cryptosporidium parvum. Previous studies have shown that the protozoan flagellate parasite Giardia duodenalis is a suitable system for the heterologous expression of secreted proteins of Apicomplexa. Here, we designed three different Cpa135 variants fused to a C-terminal HA tag in order to test their expression in G. duodenalis under the control of the inducible promoter of the cyst wall protein 1 gene (cwp1). The three Cpa135 chimeras encompassed different portions of the protein; CpaG encodes the entire polypeptide of 1574 amino acids (aa); CpaG?C includes the first 826 aa at the N-terminus; and CpaG?N consists in of the final 833 aa at the C-terminus. Immunoblot experiments showed that CpaG and CpaG?N maintained the epitopes recognized by anti-C. parvum-specific human serum. The intracellular localization and transport of the three Cpa135 variants were studied by immunofluorescence in combination with G. duodenalis-specific antibodies. CpaG?C was mainly accumulated in the endoplasmic reticulum and the intact form was also excreted in the medium. Differently, the Cpa135 chimeras possessing an intact C-terminus (CpaG and CpaG?N) were transported towards the forming cyst wall possibly and were not detected in the medium. Furthermore, the full-length CpaG was incorporated into the cyst wall. The data presented suggest that the C-terminus of Cpa135, which includes a cysteine reach domain, could influence the secretion of the chimeric proteins.lld:pubmed
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pubmed-article:21112325pubmed:statusMEDLINElld:pubmed
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pubmed-article:21112325pubmed:issn1090-2449lld:pubmed
pubmed-article:21112325pubmed:authorpubmed-author:PozioEdoardoElld:pubmed
pubmed-article:21112325pubmed:authorpubmed-author:HehlAdrian...lld:pubmed
pubmed-article:21112325pubmed:authorpubmed-author:TosiniFabioFlld:pubmed
pubmed-article:21112325pubmed:authorpubmed-author:LalleMarcoMlld:pubmed
pubmed-article:21112325pubmed:authorpubmed-author:RosatiMaria...lld:pubmed
pubmed-article:21112325pubmed:authorpubmed-author:BienJustinaJlld:pubmed
pubmed-article:21112325pubmed:copyrightInfoCopyright © 2010 Elsevier Inc. All rights reserved.lld:pubmed
pubmed-article:21112325pubmed:issnTypeElectroniclld:pubmed
pubmed-article:21112325pubmed:volume127lld:pubmed
pubmed-article:21112325pubmed:ownerNLMlld:pubmed
pubmed-article:21112325pubmed:authorsCompleteYlld:pubmed
pubmed-article:21112325pubmed:pagination680-6lld:pubmed
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pubmed-article:21112325pubmed:year2011lld:pubmed
pubmed-article:21112325pubmed:articleTitleExpression of Cryptosporidium parvum Cpa135/CpCCP1 chimeras in Giardia duodenalis: organization of the protein domains affects the protein secretion pathway.lld:pubmed
pubmed-article:21112325pubmed:affiliationDepartment of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. marco.lalle@iss.itlld:pubmed
pubmed-article:21112325pubmed:publicationTypeJournal Articlelld:pubmed