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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2011-3-7
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pubmed:abstractText |
The contribution of steroidogenic factor 1 (SF-1) to the gene expression profile of Y1 mouse adrenocortical cells was evaluated using short hairpin RNAs to knockdown SF-1. The reduced level of SF-1 RNA was associated with global changes that affected the accumulation of more than 2000 transcripts. Among the down-regulated transcripts were several with functions in steroidogenesis that were affected to different degrees--i.e., Mc2r>Scarb1>Star?Hsd3b1>Cyp11b1. For Star and Cyp11b1, the different levels of expression correlated with the amount of residual SF-1 bound to the proximal promoter regions. The knockdown of SF-1 did not affect the accumulation of Cyp11a1 transcripts even though the amount of SF-1 bound to the proximal promoter of the gene was reduced to background levels. Our results indicate that transcripts with functions in steroidogenesis vary in their dependence on SF-1 for constitutive expression. On a more global scale, SF-1 knockdown affects the accumulation of a large number of transcripts, most of which are not recognizably involved in steroid hormone biosynthesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1872-8057
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pubmed:author |
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pubmed:copyrightInfo |
Crown Copyright © 2010. Published by Elsevier Ireland Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
336
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-91
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pubmed:meshHeading |
pubmed-meshheading:21111771-Adrenal Cortex Neoplasms,
pubmed-meshheading:21111771-Animals,
pubmed-meshheading:21111771-Cell Line, Tumor,
pubmed-meshheading:21111771-Cholesterol Side-Chain Cleavage Enzyme,
pubmed-meshheading:21111771-Chromatin Immunoprecipitation,
pubmed-meshheading:21111771-Clone Cells,
pubmed-meshheading:21111771-Gene Expression Profiling,
pubmed-meshheading:21111771-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:21111771-Gene Knockdown Techniques,
pubmed-meshheading:21111771-Mice,
pubmed-meshheading:21111771-Phenotype,
pubmed-meshheading:21111771-Phosphoproteins,
pubmed-meshheading:21111771-Promoter Regions, Genetic,
pubmed-meshheading:21111771-Protein Binding,
pubmed-meshheading:21111771-RNA, Messenger,
pubmed-meshheading:21111771-RNA, Small Interfering,
pubmed-meshheading:21111771-Steroid 11-beta-Hydroxylase,
pubmed-meshheading:21111771-Steroidogenic Factor 1,
pubmed-meshheading:21111771-Steroids,
pubmed-meshheading:21111771-Transcription, Genetic,
pubmed-meshheading:21111771-Transformation, Genetic
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pubmed:year |
2011
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pubmed:articleTitle |
Contributions of steroidogenic factor 1 to the transcription landscape of Y1 mouse adrenocortical tumor cells.
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pubmed:affiliation |
Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, ON, Canada M5G 1L6. bernard.schimmer@utoronto.ca
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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