Linked Life Data

21110917

Source:http://linkedlifedata.com/resource/pubmed/id/21110917

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-11-29
pubmed:abstractText
The c-Jun NH(2)-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as JNK2?3, JNK2?4, JNK2?3, JNK2?1 and JNK2?2, respectively. Among them, JNK2?4 and JNK2?2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2?3 and JNK2?3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2?1 and JNK2?1. JNK2?1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2?3 showed higher activity on AP-1 than that of JNK2?3 and JNK2?1. Furthermore, JNK2?3 and JNK2?3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1976-670X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
738-43
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1.
pubmed:affiliation
Peking University Center for Human Disease Genomics, Beijing, 100191, P R China. sicau2000@yahoo.com.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't