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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-6-21
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pubmed:databankReference |
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pubmed:abstractText |
Lesion of the sciatic nerve caused a rapid increase in c-fos and c-jun mRNA that was followed about 2 hr later by an increase in nerve growth factor (NGF) mRNA. To evaluate whether the initial increase in c-fos mRNA is causally related to the subsequent increase in NGF mRNA, we performed experiments with fibroblasts of transgenic mice carrying an exogenous c-fos gene under the control of a metallothionein promoter. In primary cultures of these fibroblasts, CdCl2 evoked a rapid increase in exogenous c-fos mRNA, followed immediately by an increase in endogenous c-jun mRNA and with a slight delay by an increase in NGF mRNA. In fibroblasts of C3H control mice, CdCl2 had no effect on the mRNA levels of the protooncogenes c-fos and c-jun or of NGF. Additional evidence for a causal relationship between c-fos induction and the subsequent increase in NGF mRNA was obtained in cotransfection experiments. Fibroblasts of C3H control mice were cotransfected with a metallothionein-promoter-driven c-fos expression vector and a NGF promoter-chloramphenicol acetyltransferase reporter gene construct. Induction of the exogenous c-fos by CdCl2 resulted in increased activity of the NGF promoter. DNase I footprint experiments demonstrated that a binding site for transcription factor AP-1 (Fos/Jun heterodimer) in the first intron of the NGF gene was protected following c-fos induction. That this protected AP-1 site indeed was functional in the regulation of NGF expression was verified by deletion experiments and by a point mutation in the corresponding AP-1 binding region in the NGF promoter-chloramphenicol acetyltransferase reporter construct.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2431274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2457172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2462278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2464595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2511003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2542953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2570339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2744487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2761540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2786995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2821407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2825206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2839774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2840203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-2922288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3010284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3027573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3034432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3034917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3037497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3125983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3130660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3135940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3135941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3136322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3142689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3263368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3289117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3317065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3382938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3523478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3555845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3670292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3670305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-3896777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-4035354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-6278709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-6334806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-6514007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-6541297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2111020-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3899-903
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2111020-Humans,
pubmed-meshheading:2111020-Animals,
pubmed-meshheading:2111020-Mice,
pubmed-meshheading:2111020-Skin,
pubmed-meshheading:2111020-Sciatic Nerve,
pubmed-meshheading:2111020-Mutation,
pubmed-meshheading:2111020-Kinetics,
pubmed-meshheading:2111020-Fibroblasts,
pubmed-meshheading:2111020-Deoxyribonuclease I,
pubmed-meshheading:2111020-Base Sequence,
pubmed-meshheading:2111020-Cells, Cultured,
pubmed-meshheading:2111020-RNA, Messenger,
pubmed-meshheading:2111020-Nerve Growth Factors,
pubmed-meshheading:2111020-Binding Sites,
pubmed-meshheading:2111020-Organ Culture Techniques,
pubmed-meshheading:2111020-Molecular Sequence Data,
pubmed-meshheading:2111020-Promoter Regions, Genetic,
pubmed-meshheading:2111020-DNA-Binding Proteins,
pubmed-meshheading:2111020-Metallothionein
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