Linked Life Data

21108267

Source:http://linkedlifedata.com/resource/pubmed/id/21108267

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2010-12-8
pubmed:abstractText
Recently, ?-L-threofuranosyl nucleoside 3'-triphosphates (tNTPs) have been reported to be incorporated into DNA by DNA polymerases. Isonucleosides especially the 2'-deoxy-2'-isonucleosides, would be considered regioisomers of ?-L-threofuranosyl nucleosides. Therefore, we investigated the synthesis of 2'-deoxy-2'-isonucleoside 5'-triphosphates (iNTPs) having the four natural nucleobases and their incorporation into primer-template duplexes consisting of oligonucleotides containing natural 2'-deoxyribonucleosides and 2'-deoxy-2'-isonucleosides by using primer-extension reactions. We found that Klenow fragment (exo-; an A-family DNA polymerase) has strict recognition of the shape of nucleoside 5'-triphosphates and Therminator (a B-family DNA polymerase) has strict recognition of the shape of primer-template complexes, especially two base pairs upstream of the primer 3' terminus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1439-7633
pubmed:author
pubmed:issnType
Electronic
pubmed:day
10
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2597-605
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Incorporation of 2'-deoxy-2'-isonucleoside 5'-triphosphates (iNTPs) into DNA by A- and B-family DNA polymerases with different recognition mechanisms.
pubmed:affiliation
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't