Linked Life Data

2110820

Source:http://linkedlifedata.com/resource/pubmed/id/2110820

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1990-6-20
pubmed:abstractText
Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in high yields, typically in the 20-50% range. Nuclease digests of the cross-linked oligonucleotides yielded the same bifunctional MC-deoxyguanosine adduct as that previously isolated from DNA exposed to MC in vitro and in vivo [Tomasz et al. (1987) Science 235, 1204]. The cross-linked oligonucleotides displayed broad thermal melting profiles, greatly increased Tm, and complex circular dichroism spectra. Phosphodiester linkages at the cross-link were resistant to spleen exonuclease, nuclease P1, and TaqI and ClaI restriction endonucleases; snake venom diesterase action was uninhibited. The cross-links are stable to heat at neutral pH but are removed by treatment in hot piperidine or by the reducing agents Na2S2O4 and dithiothreitol. Mechanisms are proposed for these reactions. These studies define optimal methods for introducing mitomycin cross-links into DNA fragments at a specific site, providing a versatile tool to study the effects of the MC cross-links on DNA structure and function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2992-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Duplex oligodeoxyribonucleotides cross-linked by mitomycin C at a single site: synthesis, properties, and cross-link reversibility.
pubmed:affiliation
Department of Chemistry, Hunter College, City University of New York, New York 10021.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't