Source:http://linkedlifedata.com/resource/pubmed/id/21105580
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2010-11-25
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| pubmed:abstractText |
The C-terminal fragment of Clostridium perfringens enterotoxin (C-CPE) is a claudin-4 binder. Very recently, we found that nasal immunization of mice with C-CPE-fused antigen activated antigen-specific humoral and mucosal immune responses and that the deletion of the claudin-4-binding domain attenuated the immune responses. C-CPE-fusion strategy may be useful for mucosal vaccination. C-CPE is a fragment of enterotoxin, and the safety of C-CPE-fused protein is very important for its future application. In the present study, we investigated whether C-CPE-fused antigen induces immune responses without mucosal injury by using ovalbumin (OVA) as a model antigen. Immunohistochemical analysis showed that claudin-4 was expressed in epithelial cell sheets bordering the nasal cavity. Nasal immunization with C-CPE-fused OVA dose-dependently elevated the OVA-specific serum IgG titer, which was 1000-fold greater than the titer achieved by immunization with OVA or a mixture of OVA and C-CPE at 5 microg of OVA. Nasal immunization with C-CPE-fused OVA (5 microg of OVA) activated Th1 and Th2 responses. Histological analysis showed no mucosal injury in the nasal cavity or nasal passage. C-CPE-fused OVA exhibited mucosal vaccination without mucosal injury. These findings indicate thatclaudin-4-targeting using C-CPE can be a potent strategy for mucosal vaccination.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/claudin 4,
http://linkedlifedata.com/resource/pubmed/chemical/enterotoxin, Clostridium
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0031-7144
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
65
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
766-9
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| pubmed:meshHeading |
pubmed-meshheading:21105580-Administration, Intranasal,
pubmed-meshheading:21105580-Animals,
pubmed-meshheading:21105580-Clostridium Infections,
pubmed-meshheading:21105580-Clostridium perfringens,
pubmed-meshheading:21105580-Dose-Response Relationship, Immunologic,
pubmed-meshheading:21105580-Enterotoxins,
pubmed-meshheading:21105580-Female,
pubmed-meshheading:21105580-Immunity, Mucosal,
pubmed-meshheading:21105580-Immunoglobulin G,
pubmed-meshheading:21105580-Immunohistochemistry,
pubmed-meshheading:21105580-Membrane Proteins,
pubmed-meshheading:21105580-Mice,
pubmed-meshheading:21105580-Mice, Inbred BALB C,
pubmed-meshheading:21105580-Ovalbumin,
pubmed-meshheading:21105580-Peptide Fragments,
pubmed-meshheading:21105580-Th1 Cells,
pubmed-meshheading:21105580-Th2 Cells,
pubmed-meshheading:21105580-Vaccines, Subunit
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| pubmed:year |
2010
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| pubmed:articleTitle |
The safety of a mucosal vaccine using the C-terminal fragment of Clostridium perfringens enterotoxin.
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| pubmed:affiliation |
Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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