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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2010-11-24
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pubmed:abstractText |
Short hairpin RNA (shRNA) libraries are considered to comprise a powerful tool in genetic screening. Several library construction methods have been proposed; these methods require between four and nine tedious reaction steps to construct the library. In the present study, we describe a method of generating shRNA-coding DNA efficiently from randomized oligonucleotides based on four reaction steps.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1521-2254
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 John Wiley & Sons, Ltd.
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pubmed:issnType |
Electronic
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
927-33
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pubmed:meshHeading |
pubmed-meshheading:21105152-Animals,
pubmed-meshheading:21105152-Base Sequence,
pubmed-meshheading:21105152-COS Cells,
pubmed-meshheading:21105152-Cercopithecus aethiops,
pubmed-meshheading:21105152-Cloning, Molecular,
pubmed-meshheading:21105152-DNA,
pubmed-meshheading:21105152-Gene Library,
pubmed-meshheading:21105152-Genetic Vectors,
pubmed-meshheading:21105152-Inverted Repeat Sequences,
pubmed-meshheading:21105152-Luciferases, Renilla,
pubmed-meshheading:21105152-Molecular Sequence Data,
pubmed-meshheading:21105152-Plasmids,
pubmed-meshheading:21105152-RNA, Small Interfering,
pubmed-meshheading:21105152-RNA Interference,
pubmed-meshheading:21105152-Transfection
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pubmed:year |
2010
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pubmed:articleTitle |
A shRNA library constructed through the generation of loop-stem-loop DNA.
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pubmed:affiliation |
Graduate School of Bionics, Tokyo University of Technology, Hachioji, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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