Source:http://linkedlifedata.com/resource/pubmed/id/21104020
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2011-5-2
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pubmed:abstractText |
F-box proteins are key components of the ubiquitin (Ub)/26S proteasome pathway that mediates selective degradation of regulatory proteins involved in a wide variety of cellular processes affecting eukaryotic cells. In plants, F-box genes form one of the largest multigene superfamilies and control many important biological functions. Among the F-box genes characterized to date only few have been involved in the regulation of plant defense responses. Moreover, no F-box genes have been studied and characterized in grapevine. Using a differential display approach we isolated a F-box gene (BIG-24.1), which is up-regulated during Botrytis cinerea infection of grapevine leaves. BIG-24.1 encodes a polypeptide of 386 amino acids with a conserved F-box domain in the N-terminus region and a kelch domain. By investigating expression profiles of BIG-24.1, we show that the gene expression is strongly stimulated in B. cinerea infected berries and in grapevine cells challenged by MAMP rhamnolipids, a non-host bacterium and an endophytic rhizobacterium. The gene is also strongly induced by abiotic stresses including UV-C and wounding or by salicylic acid, methyl-jasmonate, ethylene and abscisic acid that are known to be involved in defense signalling pathways. In addition, sequence analysis of the BIG-24.1 promoter revealed the presence of several regulatory elements involved in the activation of plant defense responses.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1573-4978
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3327-37
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pubmed:meshHeading |
pubmed-meshheading:21104020-Amino Acid Sequence,
pubmed-meshheading:21104020-Botrytis,
pubmed-meshheading:21104020-F-Box Proteins,
pubmed-meshheading:21104020-Gene Expression Profiling,
pubmed-meshheading:21104020-Gene Expression Regulation, Plant,
pubmed-meshheading:21104020-Molecular Sequence Data,
pubmed-meshheading:21104020-Plant Growth Regulators,
pubmed-meshheading:21104020-Plant Leaves,
pubmed-meshheading:21104020-Promoter Regions, Genetic,
pubmed-meshheading:21104020-Sequence Alignment,
pubmed-meshheading:21104020-Sequence Analysis, DNA,
pubmed-meshheading:21104020-Stress, Physiological,
pubmed-meshheading:21104020-Up-Regulation,
pubmed-meshheading:21104020-Vitis
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pubmed:year |
2011
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pubmed:articleTitle |
Characterization of a F-box gene up-regulated by phytohormones and upon biotic and abiotic stresses in grapevine.
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pubmed:affiliation |
URVVC-SE-EA 2069, Stress, Défenses et Reproduction des Plantes, Université de Reims Champagne-Ardenne, BP 1039, 51687 Reims cedex 2, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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