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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2011-2-22
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pubmed:abstractText |
Rap1 is rapidly activated upon chemoattractant stimulation and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we demonstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex G?2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell movement, is activated through the G-protein coupled receptors cAR1/cAR3 and G?2 proteins in a way independent of the cGMP signaling pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/GCA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/SgcA protein, Dictyostelium...,
http://linkedlifedata.com/resource/pubmed/chemical/TERF2IP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Telomere-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/cyclic AMP receptor cAR1,
http://linkedlifedata.com/resource/pubmed/chemical/cyclic GMP-binding protein
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0219-1032
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
563-7
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pubmed:meshHeading |
pubmed-meshheading:21103944-Calcium-Binding Proteins,
pubmed-meshheading:21103944-Carrier Proteins,
pubmed-meshheading:21103944-Cell Adhesion,
pubmed-meshheading:21103944-Cell Movement,
pubmed-meshheading:21103944-Chemotactic Factors,
pubmed-meshheading:21103944-Cyclic AMP,
pubmed-meshheading:21103944-Dictyostelium,
pubmed-meshheading:21103944-GTP-Binding Protein alpha Subunits,
pubmed-meshheading:21103944-GTP-Binding Proteins,
pubmed-meshheading:21103944-Guanylate Cyclase,
pubmed-meshheading:21103944-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:21103944-Kinetics,
pubmed-meshheading:21103944-Receptors, Cyclic AMP,
pubmed-meshheading:21103944-Receptors, G-Protein-Coupled,
pubmed-meshheading:21103944-Signal Transduction,
pubmed-meshheading:21103944-Telomere-Binding Proteins
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pubmed:year |
2010
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pubmed:articleTitle |
Chemoattractant-mediated Rap1 activation requires GPCR/G proteins.
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pubmed:affiliation |
Department of Biology, College of Natural Sciences, Chosun University, Gwangju 501-759, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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