Linked Life Data

21098683

Source:http://linkedlifedata.com/resource/pubmed/id/21098683

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-2-14
pubmed:abstractText
Despite their divergent developmental ancestry, plasma cells and gastric zymogenic (chief) cells share a common function: high-capacity secretion of protein. Here we show that both cell lineages share increased expression of a cassette of 269 genes, most of which regulate endoplasmic reticulum (ER) and Golgi function, and they both induce expression of the transcription factors X-box binding protein 1 (Xbp1) and Mist1 during terminal differentiation. XBP1 is known to augment plasma cell function by establishing rough ER, and MIST1 regulates secretory vesicle trafficking in zymogenic cells. We examined morphology and function of plasma cells in wild-type and Mist1(-/-) mice and found subtle differences in ER structure but no overall defect in plasma cell function, suggesting that Mist1 may function redundantly in plasma cells. We next reasoned that MIST1 might be useful as a novel and reliable marker of plasma cells. We found that MIST1 specifically labeled normal plasma cells in mouse and human tissues, and, moreover, its expression was also characteristic of plasma cell differentiation in a cohort of 12 human plasma cell neoplasms. Overall, our results show that MIST1 is enriched upon plasma cell differentiation as a part of a genetic program facilitating secretory cell function and also that MIST1 is a novel marker of normal and neoplastic plasma cells in mouse and human tissues.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1531-2267
pubmed:author
pubmed:issnType
Electronic
pubmed:day
11
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
174-86
pubmed:dateRevised
2011-11-7
pubmed:meshHeading
pubmed-meshheading:21098683-Animals, pubmed-meshheading:21098683-Antibodies, pubmed-meshheading:21098683-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:21098683-Cell Differentiation, pubmed-meshheading:21098683-Chief Cells, Gastric, pubmed-meshheading:21098683-DNA-Binding Proteins, pubmed-meshheading:21098683-Flow Cytometry, pubmed-meshheading:21098683-Gene Expression Regulation, pubmed-meshheading:21098683-Humans, pubmed-meshheading:21098683-Intracellular Membranes, pubmed-meshheading:21098683-Leukemia, Plasma Cell, pubmed-meshheading:21098683-Lipopolysaccharides, pubmed-meshheading:21098683-Mice, pubmed-meshheading:21098683-Plasma Cells, pubmed-meshheading:21098683-Promoter Regions, Genetic, pubmed-meshheading:21098683-Syndecan-1, pubmed-meshheading:21098683-Transcription Factors
pubmed:year
2011
pubmed:articleTitle
Transcription factor MIST1 in terminal differentiation of mouse and human plasma cells.
pubmed:affiliation
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural