Linked Life Data

21096721

Source:http://linkedlifedata.com/resource/pubmed/id/21096721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-11-24
pubmed:abstractText
In drug screening and pharmaceutical research, high-throughput systems that are able to perform single-cell measurements are highly desired. Micro-electrode arrays try to answer this need but still suffer from significant drawbacks such as a small amount of electrodes and the inability to address single cells. Here, we present a novel multi-transistor array chip with 16,384 subcellular-sized electrodes based on 0.18 µm CMOS technology. We show that single-cell stimulation is possible by applying voltage pulses on the electrode to stimulate the cells lying on top. Electroporation of the cell membrane is observed using the whole-cell patch clamp technique and fluorescent dye-based live imaging. This technology could be used for high-throughput, single-cell manipulations for the purpose of large-scale drug screening and the investigation of fundamental cell processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1557-170X
pubmed:author
pubmed:issnType
Print
pubmed:volume
2010
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6473-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Single-cell stimulation and electroporation using a novel 0.18 µ CMOS chip with subcellular-sized electrodes.
pubmed:affiliation
Bioelectronics Group, Imec, Kapeldreef 75, 3001 Leuven, Belgium. dries.braeken@imec.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't