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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2011-3-9
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pubmed:abstractText |
2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-immunomics. We induced experimental autoimmune encephalomyelitis (EAE) with MBP peptide(89-104), total MBP or spinal cord homogenate. Both techniques showed anti-MBP IgG only after immunization with total MBP. In addition, 2D-immunomics revealed the presence in EAE mice of autoantibodies targeting other neural proteins, some displaying partial sequence homology with MBP. The present finding by 2D-immunomics of multiple neural proteins targeted by autoantibodies generated by a single antigen may help to explain the complex autoimmune response observed in multiple sclerosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1872-8421
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pubmed:author |
pubmed-author:AnghileriElenaE,
pubmed-author:BarbieriFrancescaF,
pubmed-author:BonettiBrunoB,
pubmed-author:CecconiDanielaD,
pubmed-author:FarinazzoAlessiaA,
pubmed-author:FurlanRobertoR,
pubmed-author:GiniBeatriceB,
pubmed-author:MarconiSilviaS,
pubmed-author:MilliAlbertoA,
pubmed-author:RuffiniFrancescaF,
pubmed-author:TuranoErmannaE
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
232
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
63-7
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pubmed:meshHeading |
pubmed-meshheading:21093930-Animals,
pubmed-meshheading:21093930-Autoantibodies,
pubmed-meshheading:21093930-Autoantigens,
pubmed-meshheading:21093930-Chromatography, High Pressure Liquid,
pubmed-meshheading:21093930-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:21093930-Encephalomyelitis, Autoimmune, Experimental,
pubmed-meshheading:21093930-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:21093930-Female,
pubmed-meshheading:21093930-Image Processing, Computer-Assisted,
pubmed-meshheading:21093930-Immunoblotting,
pubmed-meshheading:21093930-Immunoglobulin G,
pubmed-meshheading:21093930-Mass Spectrometry,
pubmed-meshheading:21093930-Mice,
pubmed-meshheading:21093930-Multiple Sclerosis,
pubmed-meshheading:21093930-Myelin Basic Proteins,
pubmed-meshheading:21093930-Peptide Fragments,
pubmed-meshheading:21093930-Spinal Cord
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pubmed:year |
2011
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pubmed:articleTitle |
2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis.
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pubmed:affiliation |
Department of Neuroscience, University of Verona, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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