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rdf:type |
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lifeskim:mentions |
umls-concept:C0036563,
umls-concept:C0205216,
umls-concept:C0205217,
umls-concept:C0599781,
umls-concept:C0997160,
umls-concept:C1159884,
umls-concept:C1257743,
umls-concept:C1555707,
umls-concept:C1705851,
umls-concept:C2752151,
umls-concept:C2828366
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pubmed:issue |
6
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pubmed:dateCreated |
2010-11-24
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pubmed:abstractText |
The aim of the present study was to investigate effects of the ethyl acetate fraction of an ethanol extract of Coix lachryma-jobi (ECLJ) on glucose uptake and adipocyte differentiation in 3T3-L1 cells. ECLJ phosphorylated AMP-activated protein kinase (AMPK) and its downstream substrate acetyl-coenzymeA carboxylase in 3T3-L1 cells in a time- and dose-dependent manner. Moreover, we discovered that compound C inhibits ECLJ-stimulated ACC phosphorylation. In addition, ECLJ exhibited a dose-dependent stimulation of glucose uptake in 3T3-L1 cells, and this increase was obviously attenuated by compound C. ECLJ also caused a decrease in the expression levels of adipogenesis factors such as fatty acid synthase, sterol-regulatory-element-binding protein-1c, peroxisome proliferator-activated receptor ?, and CAATT/enhancer binding protein ? in a dose-dependent manner. Differentiation was examined by Oil red O staining activity after ECLJ treatment for 6 days. ECLJ decreased mean droplet size. These results suggest a possible role for AMPK in the process of adipose differentiation and that ECLJ targeted for adipocyte functions could be effective in improving the symptoms of metabolic syndrome.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1557-7600
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1331-9
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pubmed:meshHeading |
pubmed-meshheading:21091246-3T3-L1 Cells,
pubmed-meshheading:21091246-AMP-Activated Protein Kinases,
pubmed-meshheading:21091246-Acetyl-CoA Carboxylase,
pubmed-meshheading:21091246-Adipocytes,
pubmed-meshheading:21091246-Adipogenesis,
pubmed-meshheading:21091246-Animals,
pubmed-meshheading:21091246-Cinnamates,
pubmed-meshheading:21091246-Coix,
pubmed-meshheading:21091246-Deoxyglucose,
pubmed-meshheading:21091246-Gene Expression Regulation,
pubmed-meshheading:21091246-Glucose,
pubmed-meshheading:21091246-Humans,
pubmed-meshheading:21091246-Mice,
pubmed-meshheading:21091246-Obesity,
pubmed-meshheading:21091246-Phenols,
pubmed-meshheading:21091246-Phosphorylation,
pubmed-meshheading:21091246-Phytotherapy,
pubmed-meshheading:21091246-Plant Extracts,
pubmed-meshheading:21091246-Protein Kinase Inhibitors,
pubmed-meshheading:21091246-Seeds,
pubmed-meshheading:21091246-Signal Transduction
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pubmed:year |
2010
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pubmed:articleTitle |
Adlay seed extract (Coix lachryma-jobi L.) decreased adipocyte differentiation and increased glucose uptake in 3T3-L1 cells.
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pubmed:affiliation |
College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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