Linked Life Data

2109032

Source:http://linkedlifedata.com/resource/pubmed/id/2109032

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-5-24
pubmed:abstractText
Cytokines such as tumor necrosis factor (TNF), interleukin-1 (IL-1), and gamma-interferon (IF) are produced by activated hematopoietic cells. They possess antiviral activity and have other biological activities such as induction of cell proliferation and hemorrhagic necrosis of tumors. Since herpes simplex virus (HSV) infection of human vascular cells is known to produce a biochemical and cytopathological effect virtually indistinguishable from atherosclerosis, we hypothesized that these cytokines many prevent cholesteryl ester (CE) accumulation in arterial smooth muscle cells (SMC) that is seen with herpesvirus infection. We now report that TNF and IL-1 but not gamma-IF prevent CE accumulation in HSV-infected arterial SMC by induction of cyclic AMP-dependent CE hydrolysis. This effect is mediated through the arachidonate 12-lipoxygenase pathway via 12-HETE since pretreatment of cells with several lipoxygenase inhibitors abolishes the antiviral effect and 12-HETE is the major (greater than 99%) lipoxygenase metabolite produced by these cells. This conclusion is further based on our observations that TNF and IL-1 enhance 12-HETE production in SMC and that 12-HETE significantly increases both intracellular cyclic AMP and lysosomal CE hydrolysis. Moreover, dibutyryl cyclic AMP restored a normal phenotype in these virally infected cells. Collectively, these findings identify for the first time a biochemical mechanism involved in the reduction of lipid accumulation in virally infected arterial SMC by these potent cytokines.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-305
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2109032-12-Hydroxy-5,8,10,14-eicosatetraenoic Acid, pubmed-meshheading:2109032-Animals, pubmed-meshheading:2109032-Arachidonate 12-Lipoxygenase, pubmed-meshheading:2109032-Arachidonate Lipoxygenases, pubmed-meshheading:2109032-Arteriosclerosis, pubmed-meshheading:2109032-Biological Factors, pubmed-meshheading:2109032-Cells, Cultured, pubmed-meshheading:2109032-Cholesterol, pubmed-meshheading:2109032-Cytokines, pubmed-meshheading:2109032-Cytopathogenic Effect, Viral, pubmed-meshheading:2109032-Drug Interactions, pubmed-meshheading:2109032-Herpes Simplex, pubmed-meshheading:2109032-Hydroxyeicosatetraenoic Acids, pubmed-meshheading:2109032-Interferon-gamma, pubmed-meshheading:2109032-Interleukin-1, pubmed-meshheading:2109032-Muscle, Smooth, Vascular, pubmed-meshheading:2109032-Tumor Necrosis Factor-alpha
pubmed:year
1990
pubmed:articleTitle
Evidence for cytokine regulation of cholesterol metabolism in herpesvirus-infected arterial cells by the lipoxygenase pathway.
pubmed:affiliation
Department of Medicine, Cornell University Medical College, New York, NY 10021.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't