Linked Life Data

21088238

Source:http://linkedlifedata.com/resource/pubmed/id/21088238

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-31
pubmed:abstractText
Patients with chemotherapy-induced gastrointestinal mucositis suffer from anorexia, diarrhea, and stomach pain, often causing weight loss and malnutrition. When the intestinal function during mucositis would be known, a rational feeding strategy might improve the nutritional state, accelerate recuperation, and increase survival of mucositis patients. We developed a methotrexate (MTX)-induced mucositis rat model to study nutrient digestion and absorption. To determine lactose digestion and absorption of its derivative glucose during mucositis, we injected Wistar rats intravenously with MTX (60 mg/kg) or 0.9% NaCl (controls). Four days later, we orally administered trace amounts of [1-(13)C]lactose and [U-(13)C]glucose and quantified the appearance of labeled glucose in the blood for 3 h. Finally, we determined plasma citrulline level and harvested the small intestine to assess histology, myeloperoxidase level, glycohydrolase activity, immunohistochemical protein, and mRNA expression. MTX-treated rats showed profound villus atrophy and epithelial damage. During the experimental period, the absorption of lactose-derived [1-(13)C]glucose was 4.2-fold decreased in MTX-treated rats compared with controls (P < 0.01). Lactose-derived [1-(13)C]glucose absorption correlated strongly with villus length (rho = 0.86, P < 0.001) and with plasma citrulline level (rho = 0.81, P < 0.001). MTX treatment decreased jejunal lactase activity (19.5-fold, P < 0.01) and immunohistochemical protein and mRNA expression (39.7-fold, P < 0.01) compared with controls. Interestingly, MTX treatment did not affect the absorption of [U-(13)C]glucose during the experimental period. We conclude that lactose digestion is severely decreased during mucositis while glucose absorption is still intact, when supplied in trace amounts. Plasma citrulline level might be a useful objective, noninvasive marker for lactose maldigestion during mucositis in clinic.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1522-1547
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G283-91
pubmed:meshHeading
pubmed-meshheading:21088238-Absorption, pubmed-meshheading:21088238-Animals, pubmed-meshheading:21088238-Citrulline, pubmed-meshheading:21088238-Digestion, pubmed-meshheading:21088238-Gastrointestinal Diseases, pubmed-meshheading:21088238-Glucose, pubmed-meshheading:21088238-Glycoside Hydrolases, pubmed-meshheading:21088238-Immunohistochemistry, pubmed-meshheading:21088238-Injections, Intravenous, pubmed-meshheading:21088238-Intestinal Mucosa, pubmed-meshheading:21088238-Jejunum, pubmed-meshheading:21088238-Lactase, pubmed-meshheading:21088238-Lactose, pubmed-meshheading:21088238-Male, pubmed-meshheading:21088238-Methotrexate, pubmed-meshheading:21088238-Microvilli, pubmed-meshheading:21088238-Mucositis, pubmed-meshheading:21088238-Pilot Projects, pubmed-meshheading:21088238-RNA, Messenger, pubmed-meshheading:21088238-Rats
pubmed:year
2011
pubmed:articleTitle
Lactose maldigestion during methotrexate-induced gastrointestinal mucositis in a rat model.
pubmed:affiliation
Pediatric Oncologist/Hematologist, Dept. of Pediatrics, Beatrix Children's Hospital/Univ. Medical Center Groningen, HPC CA21, P.O. Box 30.001, 9700 RB Groningen, The Netherlands.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't