Linked Life Data

21087080

Source:http://linkedlifedata.com/resource/pubmed/id/21087080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-10
pubmed:abstractText
The level of extracellular calcium has been demonstrated to regulate important physiological processes like cell growth and apoptosis. We demonstrate that in the bladder cancer cell line RT4, an increased extracellular calcium level induces apoptosis and that the HER1 receptor functions as a cell survival factor and delays apoptosis. After 12 h of calcium treatment (10 mM) apoptosis was detected in the RT4 cells. Increased activation of the HER1 receptor was detected as soon as 30 min after calcium addition, and the activation decreased again after 12 h of incubation, coinciding with the time when apoptosis was detectable. Inhibition of HER1 with Gefitinib (5 ?M) or Tyrphostin (AG1478) (20 ?M) augmented the calcium-induced apoptosis, and with HER1 inhibition apoptosis was detectable after 6 h. Analysis of downstream signalling molecules showed an increased activation of Akt, PLC? and MAPK in response to calcium treatment. The activation of Akt and PLC? was abolished by inhibition of HER1 with Gefitinib (5 ?M), whereas this had no effect on the activity of MAPK. In addition, incubation with inhibitors of Akt and PLC? significantly augmented calcium-induced apoptosis, whereas this was not seen with MAPK inhibition. Finally a significant increase in PKC? activity was observed with calcium treatment alone and was augmented further with HER1 inhibition. In conclusion we show that calcium-induced apoptosis in bladder cancer cells is delayed by HER1 receptor activation involving the Akt and PLC? signalling pathways.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1502-7686
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-51
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Calcium-induced apoptosis is delayed by HER1 receptor signalling through the Akt and PLC? pathways in bladder cancer cells.
pubmed:affiliation
Department of Clinical Biochemistry, NBG, AS, Aarhus University Hospital, Aarhus C, Denmark. ashfaque.a.memon@gmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't