Source:http://linkedlifedata.com/resource/pubmed/id/21084444
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-12-24
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pubmed:abstractText |
Testosterone (T) supplementation increases skeletal muscle mass, circulating GH, IGF-I, and im IGF-I expression, but the role of GH and IGF-I in mediating T's effects on the skeletal muscle remains poorly understood. Here, we show that T administration increased body weight and the mass of the androgen-dependent levator ani muscle in hypophysectomized as well as castrated plus hypophysectomized adult male rats. T stimulated the proliferation of primary human skeletal muscle cells (hSKMCs) in vitro, an effect blocked by transfecting hSKMCs with small interference RNA targeting human IGF-I receptor (IGF-IR). In differentiation conditions, T promoted the fusion of hSKMCs into larger myotubes, an effect attenuated by small interference RNA targeting human IGF-IR. Notably, MKR mice, which express a dominant negative form of the IGF-IR in skeletal muscle fibers, treated with a GnRH antagonist (acyline) to suppress endogenous T, responded to T administration by an attenuated increase in the levator ani muscle mass. In conclusion, circulating GH and IGF-I are not essential for mediating T's effects on an androgen-responsive skeletal muscle. IGF-I signaling plays an important role in mediating T's effects on skeletal muscle progenitor cell growth and differentiation in vitro. However, IGF-IR signaling in skeletal muscle fibers does not appear to be obligatory for mediating the anabolic effects of T on the mass of androgen-responsive skeletal muscles in mice.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1945-7170
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pubmed:author |
pubmed-author:BartonElisabeth RER,
pubmed-author:BhasinShalenderS,
pubmed-author:GannoMichelleM,
pubmed-author:JasujaRaviR,
pubmed-author:MorrisCarlC,
pubmed-author:SerraCarloC,
pubmed-author:ShanskyJanetJ,
pubmed-author:TangherliniFrancesF,
pubmed-author:TravisonThomas GTG,
pubmed-author:VandenburghHerman HHH,
pubmed-author:ZhangAnqiA
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pubmed:issnType |
Electronic
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
193-206
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pubmed:dateRevised |
2011-2-7
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pubmed:meshHeading |
pubmed-meshheading:21084444-Animals,
pubmed-meshheading:21084444-Body Weight,
pubmed-meshheading:21084444-Cells, Cultured,
pubmed-meshheading:21084444-Gene Expression Regulation,
pubmed-meshheading:21084444-Growth Hormone,
pubmed-meshheading:21084444-Humans,
pubmed-meshheading:21084444-Hypophysectomy,
pubmed-meshheading:21084444-Insulin-Like Growth Factor I,
pubmed-meshheading:21084444-Male,
pubmed-meshheading:21084444-Mice,
pubmed-meshheading:21084444-Muscle, Skeletal,
pubmed-meshheading:21084444-Orchiectomy,
pubmed-meshheading:21084444-RNA, Small Interfering,
pubmed-meshheading:21084444-Rats,
pubmed-meshheading:21084444-Rats, Sprague-Dawley,
pubmed-meshheading:21084444-Receptor, IGF Type 1,
pubmed-meshheading:21084444-Testosterone
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pubmed:year |
2011
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pubmed:articleTitle |
The role of GH and IGF-I in mediating anabolic effects of testosterone on androgen-responsive muscle.
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pubmed:affiliation |
Section of Endocrinology, Diabetes, and Nutrition, Boston Medical Center, 670 Albany Street, Boston, Massachusetts 02118, USA. cserra@bu.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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