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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-11-18
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pubmed:abstractText |
We aimed to develop and validate sensitive fluorescence techniques to assess the binding of magnetic microbeads derivatized with ristocetin from Amycolatopsis lurida to carboxyfluorescein-labeled D-Ala-D-Ala-D-Ala and in competition with Staphylococcus aureus bacteria. Glycopeptide antibiotics have been widely used to treat bacterial infections. However, new antibiotics are needed because of growing bacterial resistance and serious side effects. To screen potential candidates for new antibiotics, there is a great demand for sensitive, fast and inexpensive techniques to analyze the interactions between these molecules and bacterial cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1757-6199
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
721-7
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pubmed:meshHeading |
pubmed-meshheading:21083134-Anti-Bacterial Agents,
pubmed-meshheading:21083134-Bacteria,
pubmed-meshheading:21083134-Dipeptides,
pubmed-meshheading:21083134-Drug Evaluation, Preclinical,
pubmed-meshheading:21083134-Fluoresceins,
pubmed-meshheading:21083134-Fluorometry,
pubmed-meshheading:21083134-Immunoassay,
pubmed-meshheading:21083134-Limit of Detection,
pubmed-meshheading:21083134-Microspheres,
pubmed-meshheading:21083134-Peptides,
pubmed-meshheading:21083134-Ristocetin,
pubmed-meshheading:21083134-Spectrometry, Fluorescence,
pubmed-meshheading:21083134-Staphylococcus aureus
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pubmed:year |
2009
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pubmed:articleTitle |
Use of magnetic beads to study the interaction of ristocetin with peptides and bacteria.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032-98202, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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