Source:http://linkedlifedata.com/resource/pubmed/id/21082264
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2011-4-7
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| pubmed:abstractText |
A full-length metallothionein-1(MT-1) cDNA was cloned from the Chinese mitten crab, Eriocheir sinensis, based upon the hepatopancreas cDNA library. The full-length cDNA contained a single 180 bp open reading frame that encoded a 59 amino acid protein. The deduced amino acid sequence was cysteine (Cys)-rich, with residues observed in patterns characteristic of other reported MTs: Cys-X-Cys, Cys-X-X-Cys, or Cys-X-X-X-Cys. Gene structure obtained via PCR yielded a 3816 bp gene, which was comprised of three exons and two introns arranged in a "3 + 2" pattern. The cloned 5'flanking region (1,735 bp) contained several predicted binding sites, which included MREs, AP-1, SP1, USF, GATA, HNF-1, and HSF. MT-1 mRNA expression analysis revealed that while levels were highest in the hepatopancreas, expression was abundant in testis and thoracic ganglia, moderate in intestine (P<0.05), and weak in other tissues (P<0.05). MT-1 mRNA expression exhibited reproductive variation in the male, with levels approximately tenfold greater in August, during seasonal gonadal maturation, compared to other times of the year. Cu2+ exposure via tank water (0-1 mg/l for 7 days) resulted in a dose-dependent bell curve response in MT-1 mRNA expression, with peak expression observed after exposure to 0.1 mg/l Cu2+. A time course experiment (0.1 mg/l Cu2+ over 9 days) revealed MT-1 mRNA expression peaked sharply on day 5 before gradually decreasing with prolonged exposure. In the present report, we provide sequence analysis of the first MT-1 gene cloned in E. sinensis, and evidence that its physiological and toxicological regulation is evolutionary conserved.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1573-4978
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
38
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2383-93
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| pubmed:meshHeading |
pubmed-meshheading:21082264-Amino Acid Sequence,
pubmed-meshheading:21082264-Analysis of Variance,
pubmed-meshheading:21082264-Animals,
pubmed-meshheading:21082264-Base Sequence,
pubmed-meshheading:21082264-Brachyura,
pubmed-meshheading:21082264-Cloning, Molecular,
pubmed-meshheading:21082264-Copper,
pubmed-meshheading:21082264-DNA, Complementary,
pubmed-meshheading:21082264-DNA Primers,
pubmed-meshheading:21082264-Female,
pubmed-meshheading:21082264-Gene Components,
pubmed-meshheading:21082264-Gene Expression Profiling,
pubmed-meshheading:21082264-Gene Expression Regulation,
pubmed-meshheading:21082264-Male,
pubmed-meshheading:21082264-Metallothionein,
pubmed-meshheading:21082264-Molecular Sequence Data,
pubmed-meshheading:21082264-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21082264-Seasons,
pubmed-meshheading:21082264-Sequence Analysis, DNA,
pubmed-meshheading:21082264-Sex Factors
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| pubmed:year |
2011
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| pubmed:articleTitle |
Cloning, characterization, expression, and copper sensitivity of the metallothionein-1 gene in the Chinese mitten crab, Eriocheir sinensis.
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| pubmed:affiliation |
School of Life Science, East China Normal University, Shanghai, 200062, China.
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| pubmed:publicationType |
Journal Article
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