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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2010-12-24
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pubmed:abstractText |
A minimal enriched P450 BM3 library was screened for the ability to oxidize inert cyclic and acyclic alkanes. The F87A/A328V mutant was found to effectively hydroxylate cyclooctane, cyclodecane and cyclododecane. F87V/A328F with high activity towards cyclooctane hydroxylated acyclic n-octane to 2-(R)-octanol (46% ee) with high regioselectivity (92%).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1364-548X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
21
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
944-6
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pubmed:meshHeading |
pubmed-meshheading:21079837-Alkanes,
pubmed-meshheading:21079837-Bacillus megaterium,
pubmed-meshheading:21079837-Bacterial Proteins,
pubmed-meshheading:21079837-Cyclization,
pubmed-meshheading:21079837-Cytochrome P-450 Enzyme System,
pubmed-meshheading:21079837-Hydroxylation,
pubmed-meshheading:21079837-Models, Molecular,
pubmed-meshheading:21079837-Mutation,
pubmed-meshheading:21079837-NADPH-Ferrihemoprotein Reductase,
pubmed-meshheading:21079837-Oxidation-Reduction,
pubmed-meshheading:21079837-Substrate Specificity
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pubmed:year |
2011
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pubmed:articleTitle |
Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes.
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pubmed:affiliation |
Institute of Technical Biochemistry, Universitaet of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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