Linked Life Data

21079149

Source:http://linkedlifedata.com/resource/pubmed/id/21079149

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-1-28
pubmed:abstractText
In vertebrates, myeloid cells comprise polymorphonuclear and mononuclear lineages that arise from 2 successive waves of development: a transitory primitive wave giving rise to limited myeloid cells during embryonic stage and a definitive wave capable of producing myeloid cells throughout the fetal and adult life. One key unresolved question is what factors dictate polymorphonuclear versus mononuclear lineage fates during myelopoiesis. Here we show that during zebrafish embryogenesis interferon regulatory factor-8 (irf8) is expressed specifically in macrophages but not neutrophils. Suppression of Irf8 function in zebrafish causes a depletion of macrophages and an enhanced output of neutrophils but does not affect the overall number, proliferation, and survival of primitive myeloid cells. These data indicate that the skewed myeloid lineage development in Irf8 knockdown embryos results from a cell-fate switching. Such a conclusion is further supported by the observation showing that overexpression of Irf8 promotes macrophage formation at the expense of neutrophil development. Genetic epistasis analysis reveals that Irf8 acts downstream of Pu.1 but is insufficient to promote macrophage development in the absence of Pu.1. Our findings demonstrate that Irf8 is a critical determinant for neutrophil versus macrophage fate choice during zebrafish primitive myelopoiesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1528-0020
pubmed:author
pubmed:issnType
Electronic
pubmed:day
27
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1359-69
pubmed:meshHeading
pubmed-meshheading:21079149-Animals, pubmed-meshheading:21079149-Animals, Genetically Modified, pubmed-meshheading:21079149-Cell Differentiation, pubmed-meshheading:21079149-Embryo, Nonmammalian, pubmed-meshheading:21079149-Embryonic Development, pubmed-meshheading:21079149-Gene Expression Regulation, Developmental, pubmed-meshheading:21079149-Gene Knockdown Techniques, pubmed-meshheading:21079149-Green Fluorescent Proteins, pubmed-meshheading:21079149-HSP70 Heat-Shock Proteins, pubmed-meshheading:21079149-Interferon Regulatory Factors, pubmed-meshheading:21079149-Macrophages, pubmed-meshheading:21079149-Myelopoiesis, pubmed-meshheading:21079149-Neutrophils, pubmed-meshheading:21079149-Oligonucleotides, Antisense, pubmed-meshheading:21079149-Time Factors, pubmed-meshheading:21079149-Zebrafish
pubmed:year
2011
pubmed:articleTitle
Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis.
pubmed:affiliation
State Key Laboratory of Molecular Neuroscience, Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't