Linked Life Data

21076839

Source:http://linkedlifedata.com/resource/pubmed/id/21076839

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-3
pubmed:abstractText
Substance P (SP) is a multipotent neuropeptide that affects the proliferation, activation and motility of keratinocytes and fibroblasts (Fbs). SP in pulmonary and synovial cells is degraded by CD10, a 90- to 110-kDa cell surface zinc-dependent metalloprotease. However, the expression and function of CD10 in human dermal Fbs have not yet been investigated in vivo and in vitro specifically with reference to SP. Our immunohistologic study revealed moderate to strong fibroblastic CD10 expression in the majority of psoriasis vulgaris (16/16), chronic eczema (15/16), lichen planus (18/20) and atopic dermatitis (4/5). Keratinocytes showed no CD10 expression in vivo and in vitro. Cultured Fbs constitutively expressed CD10 and SP. CD10 expression was augmented by external interleukin (IL)-1? and IL-22, but not by IL-8 and IL-17A in Fbs. SP production was enhanced in CD10 knockdown-Fbs (CD10ND-Fbs) compared with control-Fbs. In the presence of IL-1? or IL-22, the enhancement of SP production was more prominent in CD10ND-Fbs than in control-Fbs, suggesting the down-modulating activity of CD10 on SP in cytokine-mediated inflammation. In conclusion, fibroblastic CD10 expression may down-regulate skin inflammation by degrading SP or reducing its level in the dermal microenvironment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1432-069X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
303
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-55
pubmed:meshHeading
pubmed-meshheading:21076839-Cell Line, pubmed-meshheading:21076839-Dermatitis, pubmed-meshheading:21076839-Down-Regulation, pubmed-meshheading:21076839-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:21076839-Fibroblasts, pubmed-meshheading:21076839-Fluorescent Antibody Technique, pubmed-meshheading:21076839-Gene Knockdown Techniques, pubmed-meshheading:21076839-Humans, pubmed-meshheading:21076839-Inflammation Mediators, pubmed-meshheading:21076839-Interleukin-1beta, pubmed-meshheading:21076839-Interleukins, pubmed-meshheading:21076839-Neprilysin, pubmed-meshheading:21076839-RNA, Small Interfering, pubmed-meshheading:21076839-RNA Interference, pubmed-meshheading:21076839-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:21076839-Skin, pubmed-meshheading:21076839-Substance P
pubmed:year
2011
pubmed:articleTitle
CD10-bearing fibroblasts may inhibit skin inflammation by down-modulating substance P.
pubmed:affiliation
Department of Dermatology, Kyushu University, Higashiku, Fukuoka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't