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pubmed-article:21074514pubmed:abstractTextThe Leu294 residue in the cytoplasmic neck of Thermotoga maritima CorA is considered to be the main gate for Mg2+ transport. We created three site-directed mutants at this position: in the Leu294Asp and Leu294Gly mutants we observed a defect in closing of the pore, while in the Leu294Arg mutant not only gating, but also the regulation of Mg2+ uptake was affected. Our results confirmed the importance of the Leu294 for gating of Mg2+ transport and in addition revealed the influence of the charge and structural features of the amino acid residues on the gating mechanism.lld:pubmed
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pubmed-article:21074514pubmed:authorpubmed-author:SchweyenRudol...lld:pubmed
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pubmed-article:21074514pubmed:copyrightInfoCopyright © 2010 Elsevier B.V. All rights reserved.lld:pubmed
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pubmed-article:21074514pubmed:volume1808lld:pubmed
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pubmed-article:21074514pubmed:pagination1587-91lld:pubmed
pubmed-article:21074514pubmed:dateRevised2011-7-28lld:pubmed
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pubmed-article:21074514pubmed:articleTitleFunctional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA.lld:pubmed
pubmed-article:21074514pubmed:affiliationDepartment of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9/4, A-1030 Vienna, Austria.lld:pubmed
pubmed-article:21074514pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21074514pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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