Linked Life Data

21073867

Source:http://linkedlifedata.com/resource/pubmed/id/21073867

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-10
pubmed:abstractText
The zebrafish pharyngeal cartilage is derived from the pharyngeal apparatus, a vertebrate-specific structure derived from all three germ layers. Developmental aberrations of the pharyngeal apparatus lead to birth defects such as Treacher-Collins and DiGeorge syndromes. While interactions between endoderm and neural crest (NC) are known to be important for cartilage formation, the full complement of molecular players involved and their roles remain to be elucidated. Activated leukocyte cell adhesion molecule a (alcama), a member of the immunoglobulin (Ig) superfamily, is among the prominent markers of pharyngeal pouch endoderm, but to date no role has been assigned to this adhesion molecule in the development of the pharyngeal apparatus. Here we show that alcama plays a crucial, non-autonomous role in pharyngeal endoderm during zebrafish cartilage morphogenesis. alcama knockdown leads to defects in NC differentiation, without affecting NC specification or migration. These defects are reminiscent of the phenotypes observed when Endothelin 1 (Edn1) signaling, a key regulator of cartilage development is disrupted. Using gene expression analysis and rescue experiments we show that Alcama functions downstream of Edn1 signaling to regulate NC differentiation and cartilage morphogenesis. In addition, we also identify a role for neural adhesion molecule 1.1 (nadl1.1), a known interacting partner of Alcama expressed in neural crest, in NC differentiation. Our data shows that nadl1.1 is required for alcama rescue of NC differentiation in edn1(-/-) mutants and that Alcama interacts with Nadl1.1 during chondrogenesis. Collectively our results support a model by which Alcama on the endoderm interacts with Nadl1.1 on NC to mediate Edn1 signaling and NC differentiation during chondrogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1095-564X
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
349
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
483-93
pubmed:dateRevised
2011-10-6
pubmed:meshHeading
pubmed-meshheading:21073867-Activated-Leukocyte Cell Adhesion Molecule, pubmed-meshheading:21073867-Animals, pubmed-meshheading:21073867-Branchial Region, pubmed-meshheading:21073867-Cell Differentiation, pubmed-meshheading:21073867-Chondrogenesis, pubmed-meshheading:21073867-Cloning, Molecular, pubmed-meshheading:21073867-DNA Primers, pubmed-meshheading:21073867-Endothelin-1, pubmed-meshheading:21073867-Gene Expression Profiling, pubmed-meshheading:21073867-Gene Knockout Techniques, pubmed-meshheading:21073867-Immunohistochemistry, pubmed-meshheading:21073867-In Situ Hybridization, Fluorescence, pubmed-meshheading:21073867-Leupeptins, pubmed-meshheading:21073867-Models, Biological, pubmed-meshheading:21073867-Neural Cell Adhesion Molecules, pubmed-meshheading:21073867-Neural Crest, pubmed-meshheading:21073867-Signal Transduction, pubmed-meshheading:21073867-Zebrafish, pubmed-meshheading:21073867-Zebrafish Proteins
pubmed:year
2011
pubmed:articleTitle
Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis.
pubmed:affiliation
Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA. priya.choudhry@hci.utah.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural