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pubmed:abstractText |
To prevent dihydroxyacetone (DHA) by-production during glyceric acid (GA) production from glycerol using Gluconobacter frateurii, we used a G. frateurii THD32 mutant, ?sldA, in which the glycerol dehydrogenase subunit-encoding gene (sldA) was disrupted, but ?sldA grew much more slowly than the wild type, growth starting after a lag of 3 d under the same culture conditions. The addition of 1% w/v D-sorbitol to the medium improved both the growth and the GA productivity of the mutant, and ?sldA produced 89.1 g/l GA during 4 d of incubation without DHA accumulation.
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pubmed:affiliation |
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. hiroshi.habe@aist.go.jp
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