Linked Life Data

21070854

Source:http://linkedlifedata.com/resource/pubmed/id/21070854

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SubjectPredicateObjectContext
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pubmed-article:21070854pubmed:dateCreated2010-12-27lld:pubmed
pubmed-article:21070854pubmed:abstractTextThe lipoglycoproteins of the WNT family act on seven transmembrane-spanning Class Frizzled receptors. Here, we show that WNT-5A evokes a proliferative response in a mouse microglia-like cell line (N13), which is sensitive to pertussis toxin, thus implicating the involvement of heterotrimeric G proteins of the G(i/o) family. We continue to show that WNT-5A stimulation of N13 membranes and permeabilized cells evokes the exchange of GDP for GTP of pertussis toxin-sensitive G proteins employing [?-(35)S]GTP assay and activity state-specific antibodies to GTP-bound G(i) proteins. Our functional analysis of the PTX-sensitivity of WNT-induced G protein activation and PCR analysis of G protein and FZD expression patterns suggest that WNT-5A stimulation leads to the activation of G(i2/3) proteins in N13 cells possibly mediated by FZD(5), the predominant FZD expressed. In summary, we provide for the first time molecular proof that WNT-5A stimulation results in the activation of heterotrimeric G(i2/3) proteins in mammalian cells with physiological protein stochiometry.lld:pubmed
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pubmed-article:21070854pubmed:authorpubmed-author:SchulteGunnar...lld:pubmed
pubmed-article:21070854pubmed:authorpubmed-author:BryjaVitezsla...lld:pubmed
pubmed-article:21070854pubmed:authorpubmed-author:KilanderMicha...lld:pubmed
pubmed-article:21070854pubmed:authorpubmed-author:DijksterhuisJ...lld:pubmed
pubmed-article:21070854pubmed:authorpubmed-author:GanjiRanjani...lld:pubmed
pubmed-article:21070854pubmed:copyrightInfoCopyright © 2010 Elsevier Inc. All rights reserved.lld:pubmed
pubmed-article:21070854pubmed:issnTypeElectroniclld:pubmed
pubmed-article:21070854pubmed:volume23lld:pubmed
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pubmed-article:21070854pubmed:pagination550-4lld:pubmed
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pubmed-article:21070854pubmed:year2011lld:pubmed
pubmed-article:21070854pubmed:articleTitleWNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins.lld:pubmed
pubmed-article:21070854pubmed:affiliationSection of Receptor Biology and Signaling, Dept. of Physiology and Pharmacology, Karolinska Institutet, S-171 77 Stockholm, Sweden.lld:pubmed
pubmed-article:21070854pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21070854pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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