| pubmed-article:21068244 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C0949575 | lld:lifeskim |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C0042774 | lld:lifeskim |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C0205132 | lld:lifeskim |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C1548602 | lld:lifeskim |
| pubmed-article:21068244 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:21068244 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:21068244 | pubmed:dateCreated | 2010-12-23 | lld:pubmed |
| pubmed-article:21068244 | pubmed:abstractText | The Rudiviridae are a family of rod-shaped archaeal viruses with covalently closed, linear double-stranded DNA (dsDNA) genomes. Their replication mechanisms remain obscure, although parallels have been drawn to the Poxviridae and other large cytoplasmic eukaryotic viruses. Here we report that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between an active-site tyrosine and the 5' end of the DNA, releasing a 3' DNA end as a primer for DNA synthesis. The enzyme can also catalyze the joining reaction that is necessary to reseal the DNA hairpin and terminate replication. The dimeric structure points to a simple mechanism through which two closely positioned active sites, each with a single tyrosine residue, work in tandem to catalyze DNA nicking and joining. We propose a novel mechanism for rudivirus DNA replication, incorporating the first known example of a Rep protein that is not linked to RCR. The implications for Rep protein function and viral replication are discussed. | lld:pubmed |
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| pubmed-article:21068244 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21068244 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21068244 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21068244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21068244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21068244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21068244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21068244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21068244 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21068244 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:21068244 | pubmed:issn | 1098-5514 | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:WhiteMalcolm... | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:NaismithJames... | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:GarrettRoger... | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:LiuHuantingH | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:PengXuX | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:PrangishviliD... | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:OkeMuseM | lld:pubmed |
| pubmed-article:21068244 | pubmed:author | pubmed-author:KerouMelinaM | lld:pubmed |
| pubmed-article:21068244 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21068244 | pubmed:volume | 85 | lld:pubmed |
| pubmed-article:21068244 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21068244 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21068244 | pubmed:pagination | 925-31 | lld:pubmed |
| pubmed-article:21068244 | pubmed:dateRevised | 2011-8-1 | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:meshHeading | pubmed-meshheading:21068244... | lld:pubmed |
| pubmed-article:21068244 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21068244 | pubmed:articleTitle | A dimeric Rep protein initiates replication of a linear archaeal virus genome: implications for the Rep mechanism and viral replication. | lld:pubmed |
| pubmed-article:21068244 | pubmed:affiliation | Biomedical Sciences Research Complex, University of St. Andrews, St. Andrews, Fife KY16 9ST, United Kingdom. | lld:pubmed |
| pubmed-article:21068244 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21068244 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:21068244 | lld:entrezgene |
