Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-12-23
pubmed:abstractText
The Rudiviridae are a family of rod-shaped archaeal viruses with covalently closed, linear double-stranded DNA (dsDNA) genomes. Their replication mechanisms remain obscure, although parallels have been drawn to the Poxviridae and other large cytoplasmic eukaryotic viruses. Here we report that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between an active-site tyrosine and the 5' end of the DNA, releasing a 3' DNA end as a primer for DNA synthesis. The enzyme can also catalyze the joining reaction that is necessary to reseal the DNA hairpin and terminate replication. The dimeric structure points to a simple mechanism through which two closely positioned active sites, each with a single tyrosine residue, work in tandem to catalyze DNA nicking and joining. We propose a novel mechanism for rudivirus DNA replication, incorporating the first known example of a Rep protein that is not linked to RCR. The implications for Rep protein function and viral replication are discussed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-10430569, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-10559333, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-10890916, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-11222090, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-11327759, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-11399079, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-11581377, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-11878892, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-12130667, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-12191478, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-15866073, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-15980588, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-16138898, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-1630899, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-16503363, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-16775310, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-16781759, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-17275023, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-17426377, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-17472345, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-17681537, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-18000036, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-18723627, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-19055817, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-19440202, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-19523475, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-20032206, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-20419351, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-2940511, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-6322422, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-7269237, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-7732000, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-7925284, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-8790393, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-8824774, http://linkedlifedata.com/resource/pubmed/commentcorrection/21068244-8864116
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1098-5514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
925-31
pubmed:dateRevised
2011-8-1
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
A dimeric Rep protein initiates replication of a linear archaeal virus genome: implications for the Rep mechanism and viral replication.
pubmed:affiliation
Biomedical Sciences Research Complex, University of St. Andrews, St. Andrews, Fife KY16 9ST, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't