Linked Life Data

21067862

Source:http://linkedlifedata.com/resource/pubmed/id/21067862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-11-30
pubmed:abstractText
MiR-34a was identified as one of the down-regulated micro-RNAs (miRs) in human colorectal cancer 5-fluorouracil (5-FU)-resistant DLD-1 cells compared with those in the parental DLD-1 cells. Exposure to 5-FU at 30 ?M activated phosphoinositide 3-kinase (PI3K)/Akt signaling markedly from 12h up to 48 h in the 5-FU-resistant cells compared with that in the parental cells and resulted in an overt difference in growth at those times. Furthermore, the expression of miR-34a in the 5-FU-resistant cells was sustained at a low-level, whereas it was up-regulated in the parental cells after the 5-FU treatment. Sirt1, which is one of the target genes for miR-34a and related to drug-resistance, was strikingly up-regulated in the 5-FU-resistant cells. The ectopic expression of miR-34a in the 5-FU-resistant cells inhibited growth, as in the parental cells, and attenuated the resistance to 5-FU through the down-regulation of Sirt1 and E2F3. Moreover, the silencing of Sirt1 significantly canceled the resistance to 5-FU in the 5-FU-resistant cells. These findings suggest that miR-34a targeting the Sirt1 and E2F3 genes could negatively regulate, at least in part, the resistance to 5-FU in human colorectal cancer DLD-1 cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1872-7980
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
28
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-204
pubmed:meshHeading
pubmed-meshheading:21067862-Antineoplastic Agents, pubmed-meshheading:21067862-Blotting, Western, pubmed-meshheading:21067862-Cell Line, Tumor, pubmed-meshheading:21067862-Drug Resistance, Neoplasm, pubmed-meshheading:21067862-E2F3 Transcription Factor, pubmed-meshheading:21067862-Fluorouracil, pubmed-meshheading:21067862-Gene Expression, pubmed-meshheading:21067862-Gene Expression Regulation, Neoplastic, pubmed-meshheading:21067862-Humans, pubmed-meshheading:21067862-In Situ Hybridization, pubmed-meshheading:21067862-MicroRNAs, pubmed-meshheading:21067862-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:21067862-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:21067862-Signal Transduction, pubmed-meshheading:21067862-Sirtuin 1, pubmed-meshheading:21067862-Transfection
pubmed:year
2011
pubmed:articleTitle
Dysregulation of microRNA-34a expression causes drug-resistance to 5-FU in human colon cancer DLD-1 cells.
pubmed:affiliation
Gifu University, Yanagido, Japan. yakao@gifu-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't