Source:http://linkedlifedata.com/resource/pubmed/id/21063882
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2011-3-3
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| pubmed:abstractText |
The aim of this study is to determine the signal transduction of membrane stretch on intermediate-conductance Ca(2+)-activated K(+) (IKca) channels in rat aorta smooth muscle cells using the patch-clamp technique. To stretch the cell membrane, both suction to the rear end of patch pipette and hypotonic shock were used. In cell-attached and inside-out patch configurations, the open probability of IKca channels increased when 20- to 45-mmHg suction was applied. Hyposmotic swelling efficiently increased IKca channel current. When the Ca(2+)-free solution was superfused, the activation of IKca current by the hyposmotic swelling was reduced. Furthermore, gadolinium (Gd(3+)) attenuated the activation of IKca channels induced by hyposmotic swelling, whereas nicardipine did not. In the experiments with Ca(2+)-free bath solution, pretreatment with GF109203X, a protein kinase C (PKC) inhibitor, completely abolished the stretch-induced activation of IKca currents. The stretch-induced activation of IKca channels was strongly inhibited by cytochalasin D, indicating a role for the F-actin in modulation of IKca channels by changes in cell stretching. These data suggest that cell membrane stretch activates IKca channels. In addition, the activation is associated with extracellular Ca(2+) influx through stretch-activated nonselective cation channels, and is also modulated by the F-actin cytoskeleton and the activation of PKC.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Intermediate-Conductance...,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1615-2573
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
26
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
91-100
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| pubmed:meshHeading |
pubmed-meshheading:21063882-Actins,
pubmed-meshheading:21063882-Animals,
pubmed-meshheading:21063882-Aorta,
pubmed-meshheading:21063882-Cell Line,
pubmed-meshheading:21063882-Cell Size,
pubmed-meshheading:21063882-Enzyme Activation,
pubmed-meshheading:21063882-Hypotonic Solutions,
pubmed-meshheading:21063882-Intermediate-Conductance Calcium-Activated Potassium...,
pubmed-meshheading:21063882-Ion Channel Gating,
pubmed-meshheading:21063882-Mechanotransduction, Cellular,
pubmed-meshheading:21063882-Membrane Potentials,
pubmed-meshheading:21063882-Muscle, Smooth, Vascular,
pubmed-meshheading:21063882-Myocytes, Smooth Muscle,
pubmed-meshheading:21063882-Osmotic Pressure,
pubmed-meshheading:21063882-Patch-Clamp Techniques,
pubmed-meshheading:21063882-Potassium Channel Blockers,
pubmed-meshheading:21063882-Protein Kinase C,
pubmed-meshheading:21063882-Protein Kinase Inhibitors,
pubmed-meshheading:21063882-Rats,
pubmed-meshheading:21063882-Time Factors
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| pubmed:year |
2011
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| pubmed:articleTitle |
Cell membrane stretch activates intermediate-conductance Ca2+-activated K+ channels in arterial smooth muscle cells.
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| pubmed:affiliation |
Department of Pediatrics, School of Medicine, University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan. hayabuchi@clin.med.tokushima-u.ac.jp
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| pubmed:publicationType |
Journal Article
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