Linked Life Data

21062977

Source:http://linkedlifedata.com/resource/pubmed/id/21062977

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2010-11-16
pubmed:abstractText
Due to the high mortality of lung cancer, there is a critical need to develop diagnostic procedures enabling early detection of the disease while at a curable stage. Targeted molecular imaging builds on the positive attributes of positron emission tomography/computed tomography (PET/CT) to allow for a noninvasive detection and characterization of smaller lung nodules, thus increasing the chances of positive treatment outcome. In this study, we investigate the ability to characterize lung tumors that spontaneously arise in a transgenic mouse model. The tumors are first identified with small animal CT followed by characterization with the use of small animal PET with a novel 64Cu-1,4,7,10-tetra-azacylododecane-N,N',N'',N'''-tetraacetic acid (DOTA)-knottin peptide that targets integrins upregulated during angiogenesis on the tumor associated neovasculature. The imaging results obtained with the knottin peptide are compared with standard 18F-fluorodeoxyglucose (FDG) PET small animal imaging. Lung nodules as small as 3 mm in diameter were successfully identified in the transgenic mice by small animal CT, and both 64Cu-DOTA-knottin 2.5F and FDG were able to differentiate lung nodules from the surrounding tissues. Uptake and retention of the 64Cu-DOTA-knottin 2.5F tracer in the lung tumors combined with a low background in the thorax resulted in a statistically higher tumor to background (normal lung) ratio compared with FDG (6.01±0.61 versus 4.36±0.68; P<0.05). Ex vivo biodistribution showed 64Cu-DOTA-knottin 2.5F to have a fast renal clearance combined with low nonspecific accumulation in the thorax. Collectively, these results show 64Cu-DOTA-knottin 2.5F to be a promising candidate for clinical translation for earlier detection and improved characterization of lung cancer.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1538-7445
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 AACR.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9022-30
pubmed:dateRevised
2011-5-4
pubmed:meshHeading
pubmed-meshheading:21062977-Animals, pubmed-meshheading:21062977-Antigens, CD31, pubmed-meshheading:21062977-Copper Radioisotopes, pubmed-meshheading:21062977-Cystine-Knot Miniproteins, pubmed-meshheading:21062977-Fluorescent Antibody Technique, pubmed-meshheading:21062977-Integrins, pubmed-meshheading:21062977-Mice, pubmed-meshheading:21062977-Mice, Transgenic, pubmed-meshheading:21062977-Mutation, pubmed-meshheading:21062977-Neoplasms, pubmed-meshheading:21062977-Neovascularization, Pathologic, pubmed-meshheading:21062977-Positron-Emission Tomography, pubmed-meshheading:21062977-Proto-Oncogene Proteins c-myc, pubmed-meshheading:21062977-Proto-Oncogene Proteins p21(ras), pubmed-meshheading:21062977-Radiopharmaceuticals, pubmed-meshheading:21062977-Sensitivity and Specificity, pubmed-meshheading:21062977-Tissue Distribution, pubmed-meshheading:21062977-Tomography, X-Ray Computed
pubmed:year
2010
pubmed:articleTitle
PET imaging of tumor neovascularization in a transgenic mouse model with a novel 64Cu-DOTA-knottin peptide.
pubmed:affiliation
Molecular Imaging Program at Stanford, Department of Radiology, Division of Oncology, Stanford University, Stanford, California 94305-5427, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural