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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-17
pubmed:abstractText
Neutral glycosphingolipids containing one to six sugars in their oligosaccharide chains have been isolated from cysts of the brine shrimp Artemia franciscana. The structures of these glycolipids were identified by methylation analysis, partial acid hydrolysis, gas-liquid chromatography, combined gas-liquid chromatography-mass spectrometry, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and proton nuclear magnetic resonance spectroscopy to be Glc?1-Cer, Man?1-4Glc?1-Cer, Fuc?1-3Man?1-4Glc?1-Cer, GlcNAc?1-3Man?1-4Glc?1-Cer, GlcNAc?1-2Fuc?1-3Man?1-4Glc?1-Cer, GalNAc?1-4GlcNAc?1-3Man?1-4Glc?1-Cer, GalNAc?1-4(Fuc?1-3)GlcNAc?1-3Man?1-4Glc?1-Cer (CPS), and GalNAc?1-4(GlcNAc?1-2Fuc?1-3)GlcNAc?1-3Man?1-4Glc?1-Cer (CHS). Two glycosphingolipids, CPS and CHS, were characterized as novel structures. Because Artemia contains a certain series of glycosphingolipids (-Fuc?3Man?4Glc?Cer), which differ from the core sugar sequences reported thus far, we tentatively designated the glycosphingolipids characterized as nonarthro-series ones. Furthermore, CHS exhibited a hybrid structure of arthro-series and nonarthro-series sugar chain. Two novel glycosphingolipids were characterized from the brine shrimp Artemia franciscana; one was composed of arthrotetraose and a branching fucose attached to N-acetylglucosamine residue, and the other was composed of CPS with an additional N-acetylglucosamine residue attached to the branching fucose.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
308-17
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Biochemical studies on sphingolipids of Artemia franciscana: novel neutral glycosphingolipids.
pubmed:affiliation
Department of Bioinformatics, Faculty of Pharmacy, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga 525-8577, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't