Linked Life Data

21061168

Source:http://linkedlifedata.com/resource/pubmed/id/21061168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-2-17
pubmed:abstractText
Iron is essential for normal brain function and its uptake in the developing rat brain peaks during the first two weeks after birth, prior to the formation of the blood–brain barrier (BBB). The first step of iron transport from the blood to the brain is transferrin receptor (TfR)-mediated endocytosis in the capillary endothelial cells. However, the subsequent step from the endothelium into interstitium has not been fully described. The goal of this study was to examine the expression of iron transport proteins by immunodetection and RT–PCR in the developing rat brain. Tf and TfR are transiently expressed in perivascular NG2+ cells of the capillary wall during the early postnatal weeks in the rat brain. However, MTP-1 and hephaestin were expressed in endothelial cells, but not in the NG2+ perivascular cells. Immunoblot analysis for these iron transfer proteins in the developing brain generally confirmed the immunochemical findings. Furthermore, the expression of Tf and TfR in the blood vessels precedes its expression in oligodendrocytes, the main iron-storing cells in the vertebrate brain. RT–PCR analysis for the primary culture of endothelial cells and pericytes revealed that Tf and TfR were highly expressed in the pericytes while MTP-1 and hephaestin were expressed in the endothelial cells. The specific expression of Tf and TfR in brain perivascular cells and MTP-1 and hephaestin in endothelial cells suggest the possibility that trafficking of elemental iron through perivascular cells may be instrumental in the distribution of iron in the developing central nervous system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1573-6830
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:21061168-Animals, pubmed-meshheading:21061168-Animals, Newborn, pubmed-meshheading:21061168-Blood-Brain Barrier, pubmed-meshheading:21061168-Brain, pubmed-meshheading:21061168-Capillaries, pubmed-meshheading:21061168-Carrier Proteins, pubmed-meshheading:21061168-Cation Transport Proteins, pubmed-meshheading:21061168-Cells, Cultured, pubmed-meshheading:21061168-Endothelial Cells, pubmed-meshheading:21061168-Gene Expression Regulation, Developmental, pubmed-meshheading:21061168-Ion Transport, pubmed-meshheading:21061168-Iron, pubmed-meshheading:21061168-Membrane Proteins, pubmed-meshheading:21061168-Rats, pubmed-meshheading:21061168-Rats, Sprague-Dawley, pubmed-meshheading:21061168-Receptors, Transferrin, pubmed-meshheading:21061168-Time Factors, pubmed-meshheading:21061168-Transferrin
pubmed:year
2011
pubmed:articleTitle
Transient expression of iron transport proteins in the capillary of the developing rat brain.
pubmed:affiliation
Department of Anatomy, Research Institute for Medical Sciences, Chungnam National University School of Medicine, Daejeon, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't