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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1990-3-29
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pubmed:abstractText |
The mouse B cell lymphoma 70Z/3 is membrane immunoglobulin M (mIgM) negative, but treatment of the cells with bacterial lipopolysaccharide (LPS) induces the expression of kappa (kappa) light chain synthesis, and the cells become mIgM+. In wild type cells, this reaction is maximal after 24 h; we have isolated a variant, 1B8, which becomes mIgM+ only after a more prolonged incubation with LPS. This delayed response results from a reduced rate of accumulation of (kappa) mRNA and protein. The transcription factor, NF-kappa B is present in the cytoplasm of both the wild type and the variant cells in its inactive form. The delay in kappa expression is correlated with the failure of NF-kappa B to be activated and translocated to the nucleus. Although NF-kappa B cannot be activated by LPS, it can be activated by treatment with phorbol ester (PMA). In contrast to the clear defect in NF-kappa B, LPS treatment of 1B8 cells causes the octamer-binding factor OTF-2 to increase normally. We conclude that the defect in 1B8 cells is in an early part of the LPS activation pathway, prior to the activation of NF-kappa B, but after the signal for OTF-2 induction. The phenotype of 1B8 demonstrates that an increase in OTF-2 alone is sufficient to cause a large increase in kappa transcription in 70Z/3 cells, but that without NF-kappa B, the response is slow to develop. In this view, NF-kappa B functions to facilitate kappa transcription and to speed its rate of increase, but is not required for the long-term response of 70Z/3 cells to LPS.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin kappa-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:issn |
0093-7711
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
65-72
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2105908-Animals,
pubmed-meshheading:2105908-B-Lymphocytes,
pubmed-meshheading:2105908-Immunoglobulin M,
pubmed-meshheading:2105908-Immunoglobulin kappa-Chains,
pubmed-meshheading:2105908-Lipopolysaccharides,
pubmed-meshheading:2105908-Lymphoma,
pubmed-meshheading:2105908-Mice,
pubmed-meshheading:2105908-NF-kappa B,
pubmed-meshheading:2105908-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2105908-Transcription, Genetic,
pubmed-meshheading:2105908-Transcription Factors
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pubmed:year |
1990
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pubmed:articleTitle |
Slow response variant of the B lymphoma 70Z/3 defective in LPS activation of NF-kappa B.
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pubmed:affiliation |
Department of Genetics SK-50, University of Washington, Seattle 98195.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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