Linked Life Data

21058714

Source:http://linkedlifedata.com/resource/pubmed/id/21058714

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2011-4-12
pubmed:abstractText
In an effort to evaluate the impact of various epidermal growth factor (EGF) grafting strategies upon cell surface receptor activation and cell adhesion, we generated low-fouling surfaces by homogeneously grafting carboxymethylated dextran (CMD) on amino-coated glass substrate. By preventing nonspecific cell adhesion while providing reactive groups facilitating subsequent protein grafting, CMD allowed achieving specific cell/tethered EGF interactions and therefore deriving unambiguous conclusions about various EGF grafting strategies. We demonstrate here that A-431 cell response to immobilized EGF is highly dependent on the bioactivity of the tagged protein being tethered, its proper orientation, and its surface density. Among all the approaches we tested, the oriented tethering of fully bioactive EGF via a de novo-designed coiled-coil capture system was shown to be the most efficient. That is, it led to the most intense and sustained phosphorylation of EGF receptors as well as to strong A-431 cell adhesion, the latter being comparable to that observed with amino-coated surfaces in the absence of CMD.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1520-4812
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2257-66
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Impact of epidermal growth factor tethering strategy on cellular response.
pubmed:affiliation
Department of Chemical Engineering, Groupe de Recherche en Sciences et Technologies Biome?dicales, Bio-P2 Research Unit, E?cole Polytechnique de Montre?al, P.O. Box 6079, succ. Centre-Ville, Montre?al (Qc), Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't