Linked Life Data

21057929

Source:http://linkedlifedata.com/resource/pubmed/id/21057929

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-11-8
pubmed:abstractText
Exocyclic etheno-DNA adducts are formed by the reaction of lipid peroxidation products, such as 4-hydroxy-2-nonenal (HNE) with DNA bases to yield 1,N (6)-etheno-2'-deoxyadenosine (?dA), 3,-N (4)-etheno-2'-deoxycytidine (?dC), and etheno-2'-deoxyguanosine. These adducts act as a driving force for many human malignancies and are elevated in the organs of cancer-prone patients suffering from chronic inflammation and infections. Here, we describe the ultrasensitive and specific techniques for the detection of ?dA and ?dC in tissue and white blood cell (WBC) DNA. This approach is based on -combined immunopurification by monoclonal antibodies and (32)P-postlabeling analysis. The detection limit is about five adducts per 10(10) parent nucleotides, requiring 5-10 ?g of DNA. In addition, we describe techniques for immunohistochemical detection of ?dA and ?dC in tissue biopsies, and the approaches for the -analysis of ?dA and ?dC excreted in urine. The utility of these detection methods for human studies is based on: (1) high sensitivity and specificity, (2) low amounts of DNA required, (3) capability to detect "background" levels of etheno-DNA adducts in biopsies, WBC, and urine samples of healthy subjects, and (4) reliable monitoring of the disease-related increase of these substances in patients.The described methods are useful in diagnosis and monitoring of chronic degenerative diseases, including cancer, atherosclerosis, and neurodegenerative disorders.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
682
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-205
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive (32)P-postlabeling and immunohistochemistry.
pubmed:affiliation
Division of Toxicology and Cancer Risk Factors, German Cancer Research Center DKFZ, Heidelberg, Germany.
pubmed:publicationType
Journal Article