Linked Life Data

21042657

Source:http://linkedlifedata.com/resource/pubmed/id/21042657

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-11-2
pubmed:abstractText
In this study, cryopreservation of Bratonia (Miltonia flavescens (Lindl.) Lindl. × Brassia longissima (Reichb.) Nash), a hybrid tropical orchid, was achieved using protocorm-like bodies (PLBs) multiplied in vitro. Cryopreservation was performed using a vitrification protocol including pretreatment of PLBs with a loading solution (LS, 2.0 M glycerol + 0.4 M sucrose) for 15 min followed by treatment with modified PVS2 vitrification solution (containing PEG instead of ethylene glycol) for 1 h. Increasing benzyladenine (BA) concentration in the recovery medium to 5.0 or 10.0 mg l?¹ during the initial 3 weeks after rewarming provided 20.4 % post-cryopreservation regrowth. By contrast, preliminary culture of PLBs with abscisic acid (ABA) and high sucrose concentrations (up to 0.3 M) as well as addition of reduced glutathione during the preculture, loading and post-culture steps were not beneficial. Forty to 45 plants were regenerated from each PLB which withstood cryopreservation. No morphological differences were observed between plants regenerated from cryopreserved and untreated PLBs. Investigations into the functional activity of photosystems I and II in PLBs suggest that electron transport was retained in the reaction centers of both photosystems shortly after cryopreservation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0143-2044
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
426-37
pubmed:meshHeading
pubmed:articleTitle
Cryopreservation of protocorm-like bodies of the hybrid orchid Bratonia (Miltonia flavescens × Brassia longissima).
pubmed:affiliation
National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Korea. elena_aygol@hotmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't