Linked Life Data

21041730

Source:http://linkedlifedata.com/resource/pubmed/id/21041730

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-11-18
pubmed:abstractText
Breast milk transmission of HIV remains an important mode of infant HIV acquisition. Enhancement of mucosal HIV-specific immune responses in milk of HIV-infected mothers through vaccination may reduce milk virus load or protect against virus transmission in the infant gastrointestinal tract. However, the ability of HIV/SIV strategies to induce virus-specific immune responses in milk has not been studied. In this study, five uninfected, hormone-induced lactating, Mamu A*01(+) female rhesus monkey were systemically primed and boosted with rDNA and the attenuated poxvirus vector, NYVAC, containing the SIVmac239 gag-pol and envelope genes. The monkeys were boosted a second time with a recombinant Adenovirus serotype 5 vector containing matching immunogens. The vaccine-elicited immunodominant epitope-specific CD8(+) T lymphocyte response in milk was of similar or greater magnitude than that in blood and the vaginal tract but higher than that in the colon. Furthermore, the vaccine-elicited SIV Gag-specific CD4(+) and CD8(+) T lymphocyte polyfunctional cytokine responses were more robust in milk than in blood after each virus vector boost. Finally, SIV envelope-specific IgG responses were detected in milk of all monkeys after vaccination, whereas an SIV envelope-specific IgA response was only detected in one vaccinated monkey. Importantly, only limited and transient increases in the proportion of activated or CCR5-expressing CD4(+) T lymphocytes in milk occurred after vaccination. Therefore, systemic DNA prime and virus vector boost of lactating rhesus monkeys elicits potent virus-specific cellular and humoral immune responses in milk and may warrant further investigation as a strategy to impede breast milk transmission of HIV.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
185
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7097-106
pubmed:dateRevised
2011-6-7
pubmed:meshHeading
pubmed-meshheading:21041730-Animals, pubmed-meshheading:21041730-Antibodies, Viral, pubmed-meshheading:21041730-DNA, Recombinant, pubmed-meshheading:21041730-DNA, Viral, pubmed-meshheading:21041730-Female, pubmed-meshheading:21041730-Gene Products, env, pubmed-meshheading:21041730-Gene Products, gag, pubmed-meshheading:21041730-Gene Products, pol, pubmed-meshheading:21041730-Genetic Vectors, pubmed-meshheading:21041730-Immunity, Cellular, pubmed-meshheading:21041730-Immunization, Secondary, pubmed-meshheading:21041730-Lactation, pubmed-meshheading:21041730-Macaca mulatta, pubmed-meshheading:21041730-Mammary Glands, Animal, pubmed-meshheading:21041730-Poxviridae, pubmed-meshheading:21041730-SAIDS Vaccines, pubmed-meshheading:21041730-Simian Acquired Immunodeficiency Syndrome, pubmed-meshheading:21041730-T-Lymphocyte Subsets, pubmed-meshheading:21041730-Vaccines, Attenuated
pubmed:year
2010
pubmed:articleTitle
Robust vaccine-elicited cellular immune responses in breast milk following systemic simian immunodeficiency virus DNA prime and live virus vector boost vaccination of lactating rhesus monkeys.
pubmed:affiliation
Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural