Source:http://linkedlifedata.com/resource/pubmed/id/21039987
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-3-22
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| pubmed:abstractText |
The aim of this study was to examine whether Nedd4L (neural precursor cell expressed, developmentally down-regulated 4-like) participated in gallbladder carcinogenesis. We first immunohistochemically examined the expression of Nedd4L in various gallbladder tissue specimens. Weak immunoreactivity to Nedd4L-specific antibody was observed in normal or dysplastic epithelial cells. Cancer cells in non-invasive regions exhibited little immunoreactivity, whereas strong immunostaining was found in cytoplasm of many invasive cancers, especially at cancer invasive front with desmoplastic reaction. Notably, siRNA-mediated silencing of the Nedd4L gene significantly decreased the Matrigel-invasion activity and collagen invasion activity of cultured gallbladder cancer cells, without affecting the cell growth. The subtractive mRNA hybridization followed by RT-PCR and immunoblotting revealed that down-regulation of Nedd4L significantly decreased the expression of collagenases, matrix metalloproteinase (MMP)-1 and -13, in gallbladder cancer cells. Finally, immunohistochemical staining showed that many Nedd4L-expressing invasive gallbladder cancer cells co-expressed MMP-1 and MMP-13. These results indicated that over-expression of Nedd4L might lead to gallbladder cancer invasion by regulating the transcription of the MMP-1 and MMP-13 genes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Endosomal Sorting Complexes...,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 13,
http://linkedlifedata.com/resource/pubmed/chemical/Nedd4 ubiquitin protein ligases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1365-2613
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| pubmed:author | |
| pubmed:copyrightInfo |
© 2010 The Authors. International Journal of Experimental Pathology © 2010 Blackwell Publishing Ltd.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
92
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
79-86
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| pubmed:meshHeading |
pubmed-meshheading:21039987-Adenocarcinoma,
pubmed-meshheading:21039987-Aged,
pubmed-meshheading:21039987-Cell Line, Tumor,
pubmed-meshheading:21039987-Down-Regulation,
pubmed-meshheading:21039987-Endosomal Sorting Complexes Required for Transport,
pubmed-meshheading:21039987-Female,
pubmed-meshheading:21039987-Gallbladder Neoplasms,
pubmed-meshheading:21039987-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:21039987-Gene Silencing,
pubmed-meshheading:21039987-Humans,
pubmed-meshheading:21039987-Immunohistochemistry,
pubmed-meshheading:21039987-Male,
pubmed-meshheading:21039987-Matrix Metalloproteinase 1,
pubmed-meshheading:21039987-Matrix Metalloproteinase 13,
pubmed-meshheading:21039987-Middle Aged,
pubmed-meshheading:21039987-Neoplasm Invasiveness,
pubmed-meshheading:21039987-RNA, Small Interfering,
pubmed-meshheading:21039987-Transcription, Genetic,
pubmed-meshheading:21039987-Transcriptional Activation,
pubmed-meshheading:21039987-Ubiquitin-Protein Ligases
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| pubmed:year |
2011
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| pubmed:articleTitle |
Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer.
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| pubmed:affiliation |
Department of Pathology, Kochi Medical School, Nankoku, Japan. takeutit08@gmail.com
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| pubmed:publicationType |
Journal Article
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