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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2011-1-19
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pubmed:abstractText |
To test the feasibility of using the survivin promoter to induce specific expression of sodium/iodide symporter (NIS) in cancer cell lines and tumors for targeted use of radionuclide therapy, a recombinant adenovirus, Ad-SUR-NIS, that expressed the NIS gene under control of the survivin promoter was constructed. Ad-SUR-NIS mediating iodide uptake and cytotoxicity was performed in vitro. Scintigraphic, biodistribution and radioiodine therapy studies were performed in vivo. PC-3 (prostate); HepG2 (hepatoma) and A375 (melanoma) cancer cells all exhibited perchlorate-sensitive iodide uptake after infection with Ad-SUR-NIS, approximately 50 times higher than that of negative control Ad-CMV-GFP-infected cells. No significant iodide uptake was observed in normal human dental pulp fibroblast (DPF) cells after infection with Ad-SUR-NIS. Clonogenic assays demonstrated that Ad-SUR-NIS-infected cancer cells were selectively killed by exposure to (131)I. Ad-SUR-NIS-infected tumors show significant radioiodine accumulation (13.3 ± 2.85% ID per g at 2 h post-injection), and the effective half-life was 3.1 h. Moreover, infection with Ad-SUR-NIS in combination with (131)I suppressed tumor growth. These results indicate that expression of NIS under control of the survivin promoter can likely be used to achieve cancer-specific expression of NIS in many types of cancers. In combination with radioiodine therapy, this strategy is a possible method of cancer gene therapy.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1476-5500
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
144-52
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pubmed:dateRevised |
2011-7-25
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pubmed:meshHeading |
pubmed-meshheading:21037556-Adenoviridae,
pubmed-meshheading:21037556-Animals,
pubmed-meshheading:21037556-Cell Line, Tumor,
pubmed-meshheading:21037556-Combined Modality Therapy,
pubmed-meshheading:21037556-Gene Therapy,
pubmed-meshheading:21037556-Humans,
pubmed-meshheading:21037556-Inhibitor of Apoptosis Proteins,
pubmed-meshheading:21037556-Iodine Radioisotopes,
pubmed-meshheading:21037556-Mice,
pubmed-meshheading:21037556-Mice, Inbred BALB C,
pubmed-meshheading:21037556-Mice, Nude,
pubmed-meshheading:21037556-Neoplasms,
pubmed-meshheading:21037556-Promoter Regions, Genetic,
pubmed-meshheading:21037556-Symporters
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pubmed:year |
2011
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pubmed:articleTitle |
Targeting of tumor radioiodine therapy by expression of the sodium iodide symporter under control of the survivin promoter.
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pubmed:affiliation |
Department of Nuclear Medicine, National Key Discipline of Medical Imaging and Nuclear Medicine, West China Hospital, Sichuan University, Chengdu, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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