Linked Life Data

21037113

Source:http://linkedlifedata.com/resource/pubmed/id/21037113

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-5
pubmed:databankReference
pubmed:abstractText
About 15% of flowering plants accumulate fructans. Inulin-type fructans with ?(2,1) fructosyl linkages typically accumulate in the core eudicot families (e.g. Asteraceae), while levan-type fructans with ?(2,6) linkages and branched, graminan-type fructans with mixed linkages predominate in monocot families. Here, we describe the unexpected finding that graminan- and levan-type fructans, as typically occurring in wheat (Triticum aestivum) and barley (Hordeum vulgare), also accumulate in Pachysandra terminalis, an evergreen, frost-hardy basal eudicot species. Part of the complex graminan- and levan-type fructans as accumulating in vivo can be produced in vitro by a sucrose:fructan 6-fructosyltransferase (6-SFT) enzyme with inherent sucrose:sucrose 1-fructosyltransferase (1-SST) and fructan 6-exohydrolase side activities. This enzyme produces a series of cereal-like graminan- and levan-type fructans from sucrose as a single substrate. The 6-SST/6-SFT enzyme was fully purified by classic column chromatography. In-gel trypsin digestion led to reverse transcription-polymerase chain reaction-based cDNA cloning. The functionality of the 6-SST/6-SFT cDNA was demonstrated after heterologous expression in Pichia pastoris. Both the recombinant and native enzymes showed rather similar substrate specificity characteristics, including peculiar temperature-dependent inherent 1-SST and fructan 6-exohydrolase side activities. The finding that cereal-type fructans accumulate in a basal eudicot species further confirms the polyphyletic origin of fructan biosynthesis in nature. Our data suggest that the fructan syndrome in P. terminalis can be considered as a recent evolutionary event. Putative connections between abiotic stress and fructans are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1532-2548
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
603-14
pubmed:meshHeading
pubmed-meshheading:21037113-Adaptation, Physiological, pubmed-meshheading:21037113-Amino Acid Sequence, pubmed-meshheading:21037113-Chromatography, Ion Exchange, pubmed-meshheading:21037113-Cloning, Molecular, pubmed-meshheading:21037113-DNA, Complementary, pubmed-meshheading:21037113-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:21037113-Evolution, Molecular, pubmed-meshheading:21037113-Freezing, pubmed-meshheading:21037113-Fructans, pubmed-meshheading:21037113-Hexosyltransferases, pubmed-meshheading:21037113-Hydrogen-Ion Concentration, pubmed-meshheading:21037113-Hydrolysis, pubmed-meshheading:21037113-Molecular Sequence Data, pubmed-meshheading:21037113-Molecular Weight, pubmed-meshheading:21037113-Pachysandra, pubmed-meshheading:21037113-Peptide Mapping, pubmed-meshheading:21037113-Phylogeny, pubmed-meshheading:21037113-Pichia, pubmed-meshheading:21037113-Sequence Alignment
pubmed:year
2011
pubmed:articleTitle
Unexpected presence of graminan- and levan-type fructans in the evergreen frost-hardy eudicot Pachysandra terminalis (Buxaceae): purification, cloning, and functional analysis of a 6-SST/6-SFT enzyme.
pubmed:affiliation
Laboratory of Molecular Plant Physiology, Institute of Botany and Microbiology, KU Leuven, B-3001 Leuven, Belgium. wim.vandenende@bio.kuleuven
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't