Linked Life Data

21036169

Source:http://linkedlifedata.com/resource/pubmed/id/21036169

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-21
pubmed:databankReference
pubmed:abstractText
Lipocalins have been known for their several biological activities in blood-sucking arthropods. Recently, the identification and characterization of lipocalins from Ixodes ricinus (LIRs) have been reported and functions of lipocalins are well documented. In this study, we have characterized four Ixodes persulcatus lipocalins that were discovered while analyzing I. persulcatus tick salivary gland EST library. We show that the four I. persulcatus lipocalins, here after named LIPERs (lipocalin from I. persulcatus) are 28.8-94.4% identical to LIRs from I. ricinus. Reverse transcriptase-PCR analysis revealed that lipocalin genes were expressed specifically in the salivary glands throughout life cycle stages of the ticks and were up-regulated by blood feeding. The specific expressions were also confirmed by Western blotting analysis. Furthermore, to investigate whether native lipocalins are secreted into the host during tick feeding, the reactivity of anti-serum raised against saliva of adult ticks to recombinant lipocalins was tested by Western blotting. The lipocalins are potentially secreted into the host during tick feeding as revealed by specific reactivity of recombinant lipocalins with mouse antibodies to I. persulcatus tick saliva. Preliminary vaccination of mice with recombinant lipocalins elicited that period to reach engorgement was significantly delayed and the engorgement weight was significantly reduced as compared to the control. Further elucidation of the biological functions of LIPERs are required to fully understand the pathways involved in the modulation of host immune responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1090-2449
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-74
pubmed:meshHeading
pubmed-meshheading:21036169-Amino Acid Sequence, pubmed-meshheading:21036169-Animals, pubmed-meshheading:21036169-Antibodies, pubmed-meshheading:21036169-Base Sequence, pubmed-meshheading:21036169-Blotting, Western, pubmed-meshheading:21036169-Cloning, Molecular, pubmed-meshheading:21036169-Cricetinae, pubmed-meshheading:21036169-DNA, Complementary, pubmed-meshheading:21036169-Feeding Behavior, pubmed-meshheading:21036169-Female, pubmed-meshheading:21036169-Gene Expression, pubmed-meshheading:21036169-Gene Expression Profiling, pubmed-meshheading:21036169-Histamine, pubmed-meshheading:21036169-Immunization, pubmed-meshheading:21036169-Ixodes, pubmed-meshheading:21036169-Lipocalins, pubmed-meshheading:21036169-Mesocricetus, pubmed-meshheading:21036169-Mice, pubmed-meshheading:21036169-Molecular Sequence Data, pubmed-meshheading:21036169-Phylogeny, pubmed-meshheading:21036169-Recombinant Proteins, pubmed-meshheading:21036169-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:21036169-Sequence Alignment, pubmed-meshheading:21036169-Specific Pathogen-Free Organisms
pubmed:year
2011
pubmed:articleTitle
Molecular identification and expression analysis of lipocalins from blood feeding taiga tick, Ixodes persulcatus Schulze.
pubmed:affiliation
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. konnai@vetmed.hokudai.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't