Linked Life Data

21030539

Source:http://linkedlifedata.com/resource/pubmed/id/21030539

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-2-7
pubmed:abstractText
Over the years several ?-glucan transferases from yeast and fungi have been reported, but enzymes with such an activity from bacteria have not been characterized so far. In this work, we describe the cloning and expression of genes encoding ?-glucosyltransferase domains of glycosyl hydrolase family GH17 from three species of proteobacteria: Pseudomonas aeruginosa PAO1, P. putida KT2440 and Azotobacter vinelandii ATCC BAA-1303. The encoded enzymes of these GH17 domains turned out to have a non-Leloir trans-?-glucosylation activity, as they do not use activated nucleotide sugar as donor, but transfer a glycosyl group from a ?-glucan donor to a ?-glucan acceptor. More particularly, the activity of the three recombinant enzymes on linear (?1 ? 3)-linked gluco-oligosaccharides (Lam-Glc(4-9)) and their corresponding alditols (Lam-Glc(4-9)-ol) was studied. Detailed structural analysis, based on thin-layer chromatography, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, electrospray ionization mass spectrometry, and 1D/2D (1)H and (13)C nuclear magnetic resonance data, revealed diverse product spectra. Depending on the enzyme used, besides (?1 ? 3)-elongation activity, (?1 ? 4)- or (?1 ? 6)-elongation, or (?1 ? 6)-branching activities were also detected.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1460-2423
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
304-28
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Exploring novel non-Leloir ?-glucosyltransferases from proteobacteria for modifying linear (?1->3)-linked gluco-oligosaccharide chains.
pubmed:affiliation
Matís Ltd, 113 Reykjavík, Iceland. gudmundur.o.hreggvidsson@matis.is
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't