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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1991-9-18
|
| pubmed:abstractText |
Melanocytic cells from white Angora goats were studied in vivo and in vitro. The histopathology of pigmented areas of skin from the most common sites of melanoma (solar-exposed areas of the ear, face, and perineum) resembled that of the epidermal melanocytes in Hutchinson's melanotic freckle in humans. Seven melanoma biopsies from 6 Angora goats showed histopathological features in common with human melanoma. A melanoma cell line, GM-1, was established in culture from a lymph node metastasis obtained from an animal that had a primary tumor excised and later developed extensive metastatic disease. GM-1 cells were mainly diploid, amelanotic, proliferated rapidly, spontaneously formed vacuolated cells, and were tumorigenic in nude mice. The species of origin of the GM-1 line was confirmed by isozyme profiles. GM-1 cultured cells and the original biopsy both expressed S-100 protein and tyrosinase antigen. Using GM-1 cells as the immunogen, a monoclonal antibody (MoAb 1F1) was derived that reacted strongly with a 116 kDa antigen in 50% of the GM-1 cells, but had little activity with goat fibroblasts (GM-F) or with human melanoma cells. GM-F, on the other hand, yielded more intense staining than GM-1 with an intermediate filament antibody (IFA), reacting with a 58 kDa antigen in both cell lines. The sensitivity of GM-1 to anticancer agents was similar to that of human melanoma cells. The pathology of caprine melanoma and its association with sun-exposed sites in relatively young animals suggest that it may be a suitable model for studying induction of melanoma by natural sunlight.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0893-5785
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
297-305
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2101929-Animals,
pubmed-meshheading:2101929-Antineoplastic Agents,
pubmed-meshheading:2101929-Cell Line,
pubmed-meshheading:2101929-Disease Models, Animal,
pubmed-meshheading:2101929-Female,
pubmed-meshheading:2101929-Goat Diseases,
pubmed-meshheading:2101929-Goats,
pubmed-meshheading:2101929-Male,
pubmed-meshheading:2101929-Melanocytes,
pubmed-meshheading:2101929-Melanoma,
pubmed-meshheading:2101929-Mice,
pubmed-meshheading:2101929-Mice, Nude,
pubmed-meshheading:2101929-Neoplasm Metastasis,
pubmed-meshheading:2101929-Neoplasm Proteins,
pubmed-meshheading:2101929-Neoplasm Transplantation,
pubmed-meshheading:2101929-Skin Neoplasms,
pubmed-meshheading:2101929-Sunlight,
pubmed-meshheading:2101929-Transplantation, Heterologous,
pubmed-meshheading:2101929-Tumor Markers, Biological
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Histopathology of melanocytic lesions in goats and establishment of a melanoma cell line: a potential model for human melanoma.
|
| pubmed:affiliation |
Queensland Institute of Medical Research, Herston, Brisbane, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|