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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1991-9-19
|
| pubmed:abstractText |
The seven members of the hok killer gene family in Gram-negative bacteria are described here. The members of this gene family have been sequenced and include hok/sok from plasmid R1, flm and srnB from plasmid F, pnd from plasmids R483 and R16, and gef and relF, which are located on the Escherichia coli chromosome. The killer proteins encoded by these loci are highly toxic polypeptides of 50 to 52 amino acids. The proteins kill the cells from the inside by interfering with a vital function in the cell membrane. On the basis of their relatedness, the killer proteins and their corresponding loci are divided into four subfamilies. The members of one subfamily, hok/sok and flm, mediate plasmid maintenance by killing plasmid-free cells. The pnd and srnB subfamilies were discovered through their abilities to cause membrane damage and degradation of stable RNA. gef and relF, which constitute the chromosomal subfamily, were found because of their sequence similarity at the DNA and protein levels with other members of the hok gene family. However, no function has been described for the proteins belonging to this subfamily. Although the four subfamilies are distantly related in terms of DNA and protein sequence similarity, the overall genetic organization of the different loci has been well conserved during evolution. The expression of all of the members of the hok gene family is regulated post-transcriptionally. Thus, the expression of the hok and flm genes is regulated by small antisense RNAs that inhibit the translation of the stable hok and flm mRNAs. On the basis of structural and functional similarities, we suggest that each of the related plasmid-encoded killer genes is regulated by antisense RNAs. The conservation of this widespread gene family in Gram-negative bacteria suggests that the genes are important to the genomes that carry them.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1043-4674
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:geneSymbol |
ccd,
cer,
flm,
flmB,
flmC,
gef,
hok,
hok/sok,
lox,
mok,
orf69,
par,
pem,
pnd,
pndA,
pndB,
pndC,
relF,
sof,
sok,
sop,
srnA,
srnB,
srnC
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
946-56
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2101633-Amino Acid Sequence,
pubmed-meshheading:2101633-Bacterial Proteins,
pubmed-meshheading:2101633-Base Sequence,
pubmed-meshheading:2101633-Cell Membrane,
pubmed-meshheading:2101633-Chromosomes, Bacterial,
pubmed-meshheading:2101633-Escherichia coli,
pubmed-meshheading:2101633-Genes, Bacterial,
pubmed-meshheading:2101633-Gram-Negative Bacteria,
pubmed-meshheading:2101633-Models, Biological,
pubmed-meshheading:2101633-Molecular Sequence Data,
pubmed-meshheading:2101633-Multigene Family,
pubmed-meshheading:2101633-Phylogeny,
pubmed-meshheading:2101633-Plasmids,
pubmed-meshheading:2101633-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2101633-Species Specificity
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
The hok killer gene family in gram-negative bacteria.
|
| pubmed:affiliation |
Department of Molecular Biology, Odense University, Denmark.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Review,
Research Support, Non-U.S. Gov't
|