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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-8-22
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pubmed:abstractText |
Effects of phosphorylation of the neurofilament L protein (NF-L) on the reassembly system were studied by both sedimentation experiments and low-angle rotary shadowing. Bovine spinal cord NF-L was phosphorylated with 3-4 mol/mol protein by either the catalytic subunit of cAMP-dependent protein kinase or protein kinase C. Phosphorylated NF-L could not assemble into filaments. Phosphorylation by either cAMP-dependent protein kinase or protein kinase C inhibited the same step of the reassembly process. Phosphorylated NF-L remained as an 8-chain complex even in favorable conditions for reassembly. The extent of the effect of phosphorylation on the filamentous structure of NF-L was also investigated by using the catalytic subunit of cAMP-dependent protein kinase. The amount of unassembled NF-L increased linearly with increased phosphorylation in the sedimentation experiments. Structural observations indicated that 1 or 2 mol of phosphorylation is enough to inhibit reassembly and to induce disassembly, and the disassembly process was also observed. The filaments were shown to unravel with disassembly. Star-like clusters, which we reported as being the initial stage of reassembly, were also identified.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-2417512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-2418034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-2465294,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-2648386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-2833525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-2839368,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-2925792,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-3172218,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-7188712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-7202005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2100199-7202006
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1044-2030
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
237-48
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
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pubmed:year |
1990
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pubmed:articleTitle |
Effects of phosphorylation of the neurofilament L protein on filamentous structures.
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pubmed:affiliation |
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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