Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-11-24
pubmed:abstractText
Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this method for siRNA delivery, we achieve >90% reduction in the expression of the stem cell factors Oct4 and Lin28, and observe cell morphological and staining pattern changes, characteristics of hESC differentiation, as a result of Oct4 knockdown.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-15749924, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-16632596, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-17355893, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-17529971, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-18029452, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-18035408, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-18157870, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-18323409, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-18819918, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-19235204, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-19443445, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-19966271, http://linkedlifedata.com/resource/pubmed/commentcorrection/20978109-9804556
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1469-9001
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2564-9
pubmed:dateRevised
2011-7-28
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
High-efficiency siRNA-based gene knockdown in human embryonic stem cells.
pubmed:affiliation
Yale Stem Cell Center, New Haven, Connecticut 06520, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies