|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2010-11-29
|
|
pubmed:abstractText |
Circulating tumor cells (CTC) have been identified in several human malignancies, including malignant melanoma. However, whether melanoma CTC are tumorigenic and cause metastatic progression is currently unknown. Here, we isolate for the first time viable tumorigenic melanoma CTC and demonstrate that this cell population is capable of metastasis formation in human-to-mouse xenotransplantation experiments. The presence of CTC among peripheral blood mononuclear cells (PBMC) of murine recipients of subcutaneous (s.c.) human melanoma xenografts could be detected based on mRNA expression for human GAPDH and/or ATP-binding cassette subfamily B member 5 (ABCB5), a marker of malignant melanoma-initiating cells previously shown to be associated with metastatic disease progression in human patients. ABCB5 expression could also be detected in PBMC preparations from human stage IV melanoma patients but not healthy controls. The detection of melanoma CTC in human-to-mouse s.c. tumor xenotransplantation models correlated significantly with pulmonary metastasis formation. Moreover, prospectively isolated CTC from murine recipients of s.c. melanoma xenografts were capable of primary tumor initiation and caused metastasis formation upon xenotransplantation to secondary murine NOD-scid IL2R?(null) recipients. Our results provide initial evidence that melanoma CTC are tumorigenic and demonstrate that CTC are capable of causing metastatic tumor progression. These findings suggest a need for CTC eradication to inhibit metastatic progression and provide a rationale for assessment of therapeutic responses of this tumorigenic cell population to promising emerging melanoma treatment modalities.
|
|
pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/1R01CA113796,
http://linkedlifedata.com/resource/pubmed/grant/1R01CA138231,
http://linkedlifedata.com/resource/pubmed/grant/2P50CA093683,
http://linkedlifedata.com/resource/pubmed/grant/P50 CA093683-07,
http://linkedlifedata.com/resource/pubmed/grant/P50 CA093683-08,
http://linkedlifedata.com/resource/pubmed/grant/P50 CA093683-09,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA113796-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA113796-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA113796-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA113796-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA113796-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA138231-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA138231-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA138231-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 CA138231-04
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
1090-2104
|
|
pubmed:author |
pubmed-author:AllooAllirezaA,
pubmed-author:FrankMarkus HMH,
pubmed-author:FrankNatasha YNY,
pubmed-author:GasserMartinM,
pubmed-author:LinJennifer YJY,
pubmed-author:LobMM,
pubmed-author:MurphyGeorge FGF,
pubmed-author:SchattonTobiasT,
pubmed-author:Stephen HodiFF,
pubmed-author:Waaga-GasserAna-MariaAM,
pubmed-author:WilsonBrian JBJ,
pubmed-author:ZhanQianQ
|
|
pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
26
|
|
pubmed:volume |
402
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
711-7
|
|
pubmed:dateRevised |
2011-10-6
|
|
pubmed:meshHeading |
pubmed-meshheading:20977885-Animals,
pubmed-meshheading:20977885-Cell Separation,
pubmed-meshheading:20977885-Cell Transformation, Neoplastic,
pubmed-meshheading:20977885-Humans,
pubmed-meshheading:20977885-Melanoma,
pubmed-meshheading:20977885-Mice,
pubmed-meshheading:20977885-Mice, Nude,
pubmed-meshheading:20977885-Neoplasm Metastasis,
pubmed-meshheading:20977885-Neoplasm Transplantation,
pubmed-meshheading:20977885-Neoplastic Cells, Circulating,
pubmed-meshheading:20977885-P-Glycoprotein,
pubmed-meshheading:20977885-Skin Neoplasms,
pubmed-meshheading:20977885-Tumor Markers, Biological
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Isolation of tumorigenic circulating melanoma cells.
|
|
pubmed:affiliation |
Transplantation Research Center, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
